Excessive Extracellular Ammonium Production by a Free-Living Nitrogen-Fixing Soil Clostridium sp. Strain.

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Soyeon Park, Jeonghwan Jang
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引用次数: 0

Abstract

A Gram-positive, rod-shaped, and obligate anaerobic bacterial strain OS1-26 was isolated from apple orchard soil in Iksan, South Korea. Interestingly, strain OS1-26 was observed to possess the functional genes involved in biological nitrogen fixation (BNF), including nifH, which was actively transcribed during the anaerobic cultivation with excessive production of extracellular NH4+ despite of presence of other fixed N nutrients. The BNF of strain OS1-26 was distinguished from the other well-known Clostridium diazotrophs, such as C. pasteurianum and C. acetobutylicum. The altruistic N-fixing ability of the strain may play a pivotal role in providing N nutrients to the microbial community and plants in the soil ecosystem. The microorganism grew at 25-35 °C (optimum 30-35 °C) and pH 5.0-8.0 (optimum 6.0-8.0) but was not able to grow in the presence of >0.5% NaCl. The major cellular fatty acids of strain OS1-26 were C16:0, C14:0, and the summed feature consisted of C16:1 ω7c and C16:1 ω6c (35.63%, 25.29%, and 18.84%, respectively). The 16S rRNA phylogeny indicated that strain OS1-26 is a member of the genus Clostridium, and the closest species are C. aciditolerans, C. nitrophenolicum, and C. thailandense, with 16S rRNA sequence similarities such as 99.71%, 98.52%, and 98.45%, respectively. In spite of the high 16S rRNA sequence similarity, strain OS1-26 showed overall genomic relatedness, such as the average nucleotide identity (ANI), and phenotypical features distinctly different from Clostridium aciditolerans. Although the species taxonomy of strain OS1-26 is undetermined within the genus Clostridium based on overall genomic and phenotypic properties, further studies on the soil bacterial strain would enhance our understanding of its taxonomic identity, ecological roles for the terrestrial soil N cycle, and the potential to be developed as a biological N fertilizer.

游离固氮土壤梭状芽胞杆菌胞外过量产铵的研究。
从韩国益山苹果园土壤中分离到一株革兰氏阳性棒状专性厌氧细菌OS1-26。有趣的是,菌株OS1-26被观察到具有参与生物固氮(BNF)的功能基因,包括nifH,该基因在厌氧培养过程中活跃转录,尽管存在其他固定N营养物质,但细胞外NH4+的过量生产。菌株OS1-26的BNF与其他已知的重氮养梭菌(如C. pasteuranum和C. acetobutylicum)有明显区别。该菌株的利他固氮能力可能在向土壤生态系统中的微生物群落和植物提供氮养分方面发挥关键作用。该微生物在25 ~ 35℃(最适30 ~ 35℃)和pH 5.0 ~ 8.0(最适6.0 ~ 8.0)条件下生长,但在>0.5% NaCl存在下不能生长。菌株OS1-26的主要细胞脂肪酸为C16:0、C14:0,其总特征为C16:1 ω7c和C16:1 ω6c(分别为35.63%、25.29%和18.84%)。16S rRNA系统发育结果表明,菌株OS1-26属于梭状芽孢杆菌属,与菌株C. aciditolerans、C. nitrophenolicum和C.泰国梭状芽孢杆菌的16S rRNA序列相似性分别为99.71%、98.52%和98.45%。尽管菌株OS1-26具有较高的16S rRNA序列相似性,但菌株OS1-26在平均核苷酸同源性(ANI)和表型特征等方面与嗜酸梭菌具有明显的差异。虽然菌株OS1-26在Clostridium属中的物种分类尚未确定,但对该土壤细菌菌株的进一步研究将有助于我们了解其分类特征、对陆地土壤氮循环的生态作用以及开发作为生物氮肥的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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