The influence of probiotic administration on selected leukocyte subpopulations and the serum amyloid A concentration in the peripheral blood of dairy cows during different lactation periods.

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Research Pub Date : 2024-10-09 eCollection Date: 2024-12-01 DOI:10.2478/jvetres-2024-0057

Piotr Brodzki, Jan Marczuk, Hubert Gorzkoś, Urszula Lisiecka, Marek Szczubiał, Adam Brodzki, Roman Dąbrowski, Leszek Krakowski, Katarzyna Głodkowska, Nikodem Brodzki

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Abstract

Introduction: The aim of the study was to compare selected leukocyte subpopulations and the serum amyloid A (SAA) concentration in the peripheral blood of cows at different stages of lactation. The blood of cows receiving a probiotic as a dietary supplement was compared with the blood of cows not receiving it.

Material and methods: The research was conducted on 20 pregnant dairy cows randomly divided into two groups of 10 cows each. The experimental group consisted of cows given the probiotic as a feed supplement. The control group consisted of cows that were fed without supplementation. Blood was drawn six times for testing: 7 days before drying; 14 days before parturition; and 7, 21, 60 and 90 days postpartum. Leukocyte immunophenotyping was performed by flow cytometry.

Results: The blood of cows administered the probiotic revealed an increased percentage of forkhead box protein 3 (Foxp3)⁺, T CD4⁺ and B CD25⁺ lymphocytes and β2 CD18⁺ and αM CD11b⁺ integrins, and persistently low SAA levels at all time points.

Conclusion: The activity of the immune system in cows receiving the probiotic was higher than in control cows. However, the stabilisation of the immune system of the supplemented cows may be indicated by the persistence of a low level of SAA throughout the experiment. Therefore, it can be assumed that the immune system of cows treated with the probiotic more easily adapts to changes in conditions in particular lactation periods and that these cows become more resistant to infectious diseases.

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不同泌乳期益生菌对奶牛外周血白细胞亚群及血清淀粉样蛋白A浓度的影响

前言：本研究的目的是比较不同泌乳期奶牛外周血中选定白细胞亚群和血清淀粉样蛋白A （SAA）浓度。将接受益生菌作为膳食补充剂的奶牛的血液与未接受益生菌的奶牛的血液进行比较。材料与方法：选用20头妊娠奶牛，随机分为两组，每组10头。试验组为以益生菌作为饲料补充的奶牛。对照组为不添加饲料的奶牛。抽血6次检测：干燥前7天；产前14天；以及产后7、21、60和90天。流式细胞术进行白细胞免疫分型。结果：饲喂益生菌的奶牛血液中Foxp3 +、T CD4+和B CD25+淋巴细胞以及β2 CD18+和αM CD11b+整合素的比例均升高，SAA水平在各时间点均持续降低。结论：饲喂益生菌的奶牛免疫系统活性明显高于对照组奶牛。然而，在整个试验过程中，低水平SAA的持续存在可能表明补充奶牛免疫系统的稳定。因此，可以认为，奶牛的免疫系统更容易适应特定哺乳期条件的变化，并且这些奶牛对传染病的抵抗力更强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Research Veterinary-General Veterinary

CiteScore

0.90

自引率

5.60%

发文量

审稿时长

18 weeks

期刊介绍： Journal of Veterinary Research (formerly Bulletin of the Veterinary Institute in Pulawy) is a quarterly that publishes original papers, review articles and short communications on bacteriology, virology, parasitology, immunology, molecular biology, pathology, toxicology, pharmacology, and biochemistry. The main emphasis is, however, on infectious diseases of animals, food safety and public health, and clinical sciences.