Anti-inflammatory effect of sea buckthorn in an HCl-induced cystitis rat model.

IF 2.5 3区医学 Q2 UROLOGY & NEPHROLOGY

Investigative and Clinical Urology Pub Date : 2025-01-01 DOI:10.4111/icu.20240196

Hyun Suk Yoon, Juyeon Yu, Shinhoon Kang, Hana Yoon

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引用次数: 0

Abstract

Purpose: Although the mechanism underlying interstitial cystitis/bladder pain syndrome (IC/BPS) remains unclear, oxidative stress is suggested to be implicated in IC/BPS development. Sea buckthorn (SB; Hippophae rhamnoides L.) contains several compounds with antioxidant properties. In addition, intravesical application of hydrochloric acid (HCl) in rats induces histological changes similar to those observed in humans with IC. Therefore, the aim of this study was to evaluate the anti-inflammatory effects of SB in an HCl-induced rat cystitis model.

Materials and methods: Twenty 8-week-old female Sprague-Dawley rats were instilled with HCl in their bladders to create an IC/BPS model. The model rats were divided into three groups and orally administrated distilled water (control, n=4), concentrated SB (n=8), or pentosan polysulfate (PPS, n=8) daily. Pathologic inflammation grade (H&E staining), number of mast cells per square millimeter (toluidine blue staining), fibrotic changes (Masson's trichrome staining), and apoptosis (terminal deoxynucleotidyl transferase dUTP nick end labeling staining) of bladder tissue samples were compared among the groups.

Results: Compared to the control group, the SB and PPS groups showed reduced edema (5.25±0.96 vs. 2.25±0.46 vs. 2.50±0.54, p=0.004, p=0.005, respectively), number of mast cells (12.5±3.6 vs. 6.8±1.9 vs. 6.6±1.8, p=0.010, p=0.002, respectively), ratio of fibrotic submucosal tissue (63.9%±7.0% vs. 43.6%±9.9% vs. 40.5%±5.2%, p<0.001, p<0.001, respectively), and ratio of apoptotic nucleus (40.7%±11.7% vs. 7.7%±6.5% vs. 5.1%±4.9%, p<0.001, p<0.001, respectively).

Conclusions: SB exhibited anti-inflammatory effects comparable to those of PPS in the HCl-induced chemical cystitis model.

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沙棘对盐酸致膀胱炎大鼠模型的抗炎作用。

目的：虽然间质性膀胱炎/膀胱痛综合征（IC/BPS）的机制尚不清楚，但氧化应激提示与IC/BPS的发展有关。沙棘；沙棘（Hippophae rhamnoides L.）含有几种具有抗氧化特性的化合物。此外，大鼠膀胱内应用盐酸（HCl）可诱导与IC患者相似的组织学变化。因此，本研究的目的是在盐酸诱导的大鼠膀胱炎模型中评估SB的抗炎作用。材料与方法：将20只8周龄雌性sd大鼠膀胱灌注HCl，建立IC/BPS模型。将模型大鼠分为3组，每天口服蒸馏水（对照组，n=4）、浓缩SB （n=8）、聚硫酸戊聚糖（PPS, n=8）。比较各组膀胱组织病理炎症分级（H&E染色）、每平方毫米肥大细胞数（甲苯胺蓝染色）、纤维化改变（马松三色染色）、凋亡（末端脱氧核苷酸转移酶dUTP nick末端标记染色）。结果：与对照组比较，SB组和PPS组水肿减少（5.25±0.96比2.25±0.46比2.50±0.54,p=0.004, p=0.005），肥大细胞数量减少（12.5±3.6比6.8±1.9比6.6±1.8,p=0.010, p=0.002），粘膜下组织纤维化比例减少（63.9%±7.0%比43.6%±9.9%比40.5%±5.2%），结论：SB对盐酸致化学性膀胱炎模型的抗炎作用与PPS相当。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Investigative and Clinical Urology Medicine-Urology

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

4 weeks

期刊介绍： Investigative and Clinical Urology (Investig Clin Urol, ICUrology) is an international, peer-reviewed, platinum open access journal published bimonthly. ICUrology aims to provide outstanding scientific and clinical research articles, that will advance knowledge and understanding of urological diseases and current therapeutic treatments. ICUrology publishes Original Articles, Rapid Communications, Review Articles, Special Articles, Innovations in Urology, Editorials, and Letters to the Editor, with a focus on the following areas of expertise: • Precision Medicine in Urology • Urological Oncology • Robotics/Laparoscopy • Endourology/Urolithiasis • Lower Urinary Tract Dysfunction • Female Urology • Sexual Dysfunction/Infertility • Infection/Inflammation • Reconstruction/Transplantation • Geriatric Urology • Pediatric Urology • Basic/Translational Research One of the notable features of ICUrology is the application of multimedia platforms facilitating easy-to-access online video clips of newly developed surgical techniques from the journal''s website, by a QR (quick response) code located in the article, or via YouTube. ICUrology provides current and highly relevant knowledge to a broad audience at the cutting edge of urological research and clinical practice.