Effect of Eugenol on Detrusor Muscle: Potential for Overactive Bladder Treatment.

IF 1.8 3区医学 Q3 UROLOGY & NEPHROLOGY

International Neurourology Journal Pub Date : 2024-12-01 Epub Date: 2024-12-31 DOI:10.5213/inj.2448326.163

Marília Cavalcante Araújo, Átila Pereira-Gonçalves, André Nogueira Cardeal Dos Santos, José Ednésio da Cruz Freire, Luís Peireira-de-Morais, Francisco Sydney Henrique-Félix, Nicoly Câmara Castro Sousa-Júlio, José Henrique Leal-Cardoso, Andrelina Noronha Coelho-de-Souza

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引用次数: 0

Abstract

Purpose: This investigation was conducted to elucidate the effects of eugenol on bladder contractility through experimental and in silico approaches.

Methods: To assess the impact of eugenol on muscular contractility, longitudinal strips of bladder tissue, measuring 2 mm by 6 mm, were mounted in perfusion chambers connected to an isometric force transducer. Furthermore, molecular docking studies were conducted to explore the potential of eugenol to target the M3 muscarinic acetylcholine receptor (M3R) and voltage-operated calcium channels (VOCCs) in muscle cells, utilizing in silico techniques.

Results: Eugenol exhibited a concentration-dependent inhibitory effect on both the phasic and tonic components of the contraction induced by 60mM K+ and carbachol, completely suppressing this contraction at a concentration of 3mM. Additionally, eugenol inhibited the concentration-contraction curve elicited by Ba2+.

Conclusion: The in vitro and in silico results suggest that the mechanism of eugenol likely involves blockade of VOCCs and/or M3R, implicating eugenol as a promising molecule for the treatment of overactive bladder.

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丁香酚对逼尿肌的作用：治疗膀胱过度活动的潜力。

目的：通过实验和计算机方法研究丁香酚对膀胱收缩力的影响。方法：为了评估丁香酚对肌肉收缩力的影响，将2 × 6 mm的膀胱组织纵条置于与等距力传感器相连的灌注室中。此外，利用硅技术进行了分子对接研究，以探索丁香酚靶向肌肉细胞中M3毒菌碱乙酰胆碱受体（M3R）和电压操作钙通道（vocc）的潜力。结果：丁香酚对60mM K+和carbachol诱导的收缩的相性和紧张性成分均表现出浓度依赖的抑制作用，在3mM浓度下完全抑制这种收缩。丁香酚抑制Ba2+诱导的浓度-收缩曲线。结论：丁香酚的体外和体内实验结果提示丁香酚的作用机制可能与阻断VOCCs和/或M3R有关，丁香酚是治疗膀胱过动症的有前景的分子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Neurourology Journal UROLOGY & NEPHROLOGY-

CiteScore

4.40

自引率

21.70%

发文量

审稿时长

4 weeks

期刊介绍： The International Neurourology Journal (Int Neurourol J, INJ) is a quarterly international journal that publishes high-quality research papers that provide the most significant and promising achievements in the fields of clinical neurourology and fundamental science. Specifically, fundamental science includes the most influential research papers from all fields of science and technology, revolutionizing what physicians and researchers practicing the art of neurourology worldwide know. Thus, we welcome valuable basic research articles to introduce cutting-edge translational research of fundamental sciences to clinical neurourology. In the editorials, urologists will present their perspectives on these articles. The original mission statement of the INJ was published on October 12, 1997. INJ provides authors a fast review of their work and makes a decision in an average of three to four weeks of receiving submissions. If accepted, articles are posted online in fully citable form. Supplementary issues will be published interim to quarterlies, as necessary, to fully allow berth to accept and publish relevant articles.