Four novel nontuberculous mycobacteria species: Mycobacterium wendilense sp. nov., Mycobacterium burgundiense sp. nov., Mycobacterium kokjensenii sp. nov. and Mycobacterium holstebronense sp. nov. revived from a historical Danish strain collection.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-01-01 DOI:10.1099/ijsem.0.006620

Xenia Emilie Sinding Iversen, Erik Michael Rasmussen, Dorte Bek Folkvardsen, Erik Svensson, Conor J Meehan, René Jørgensen, Anders Norman, Troels Lillebaek

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Abstract

Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S rrs, internal transcribed spacer and 23S rrl elements, as well as a single-copy core-gene (hsp65, rpoB+C, secA and tuf) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains. A genomic comparison to the closest related type strain was done by calculating average nucleotide identity and in silico DNA:DNA hybridization values, which showed 87.9% and 33.0% for Mu0050, 85.2% and 27.4% for Mu0053, 85.3% and 27.6% for Mu0083 and 93.3% and 50.1% for Mu0102, respectively. The names proposed for the new species are Mycobacterium wendilense sp. nov. (Mu0050^T=ITM 501390^T=CCUG 77525^T), Mycobacterium burgundiense sp. nov. (Mu0053^T=ITM 501391^T=CCUG 77526^T), Mycobacterium kokjensenii sp. nov. (Mu0083^T=ITM 501392^T=CCUG 77527^T) and Mycobacterium holstebronense sp. nov. (Mu0102^T=ITM 501393^T=CCUG 77528^T).

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四种新的非结核分枝杆菌：文迪氏分枝杆菌，勃艮第分枝杆菌，kokjensenii分枝杆菌和holstebronense分枝杆菌，从丹麦的历史菌株收集中复活。

在丹麦哥本哈根Statens血清研究所国际分枝杆菌学参考实验室的历史菌株收集中发现了四种新的非结核分枝杆菌。对1948 ~ 1955年间临床从胃灌洗液中分离的冻干分枝杆菌进行了16S rrs、内部转录间隔段和23S rrl元件以及一个单拷贝核心基因（hsp65、rpoB+C、secA和tuf）的系统发育分析，结果表明，这些分枝杆菌与Terra和Fortuitum-Vaccae分支的类型菌株分组有关。表型特征、生化特性以及脂肪酸和霉菌酸谱支持其分类为新菌株。结果表明，Mu0050、Mu0053、Mu0083和Mu0102分别为87.9%和33.0%、85.2%和27.4%、85.3%和27.6%、93.3%和50.1%。提出的新种名称为温氏分枝杆菌sp. 11 （Mu0050T=ITM 501390T=CCUG 77525T）、勃艮第分枝杆菌sp. 11 （Mu0053T=ITM 501391T=CCUG 77526T）、柯延森分枝杆菌sp. 11 （Mu0083T=ITM 501392T=CCUG 77527T）和霍尔斯特bronense分枝杆菌sp. 11 （Mu0102T=ITM 501393T=CCUG 77528T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.