Immunomodulatory Effects of Pterocarpanquinone LQB-118 in Murine Peritoneal Macrophages.

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunological Investigations Pub Date : 2025-01-08 DOI:10.1080/08820139.2025.2449949

Éssia de Almeida Lima, Luiz Henrique Agra Cavalcante-Silva, Deyse Cristina Madruga Carvalho, Mariana Mendonça Soares, Anna Beatriz Araujo Medeiros, Chaquip Daher Netto, Paulo Roberto Ribeiro Costa, Sandra Rodrigues-Mascarenhas

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引用次数: 0

Abstract

Background: Phagocytosis is an important function of macrophages. However, when it's dysregulated, it could compromise homeostasis. Thus, this study aimed to assess the inhibitory activity of pterocarpanquinone LQB 118 on murine macrophage phagocytosis.

Methods: We used peritoneal macrophages isolated from mice to evaluate the impact of LQB 118 (5 μM) on the modulation of phagocytic activity and possible action mechanism related: IL-12 (by ELISA), NO (by Griess reaction),ROS production (by flow cytometry), and intracellular signaling proteins (iNOS, P-Akt, P-mTOR, NF-κB, and P-NF-κB) (by flow cytometry).The macrophages were stimulated with zymosan to assess both phagocytic activity and flow cytometry assays.

Results: Treatment with LQB 118 resulted in a reduction in the phagocytosis of zymosan particles by macrophages. This effect could potentially be attributed to LQB's inhibition of IL-12 production and mTOR/NF-κB signaling. Furthermore, LQB 118 decreased the levels of ROS and NO without interfering with iNOS and Akt activation.

Conclusion: These findings show the anti-phagocytic activity of LQB 118 on macrophage, highlighting the potential of this compound as a candidate for modulating macrophage-driven inflammation.

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pterocarpan醌LQB-118对小鼠腹腔巨噬细胞的免疫调节作用。

背景：吞噬是巨噬细胞的一项重要功能。然而，当它失调时，它可能会破坏体内平衡。因此，本研究旨在评价紫杉卡潘醌lqb118对小鼠巨噬细胞吞噬的抑制作用。方法：采用小鼠腹腔巨噬细胞，观察lqb118 （5 μM）对巨噬细胞吞噬活性的调节作用及可能的作用机制：IL-12 （ELISA）、NO （Griess反应）、ROS生成（流式细胞术）和细胞内信号蛋白（iNOS、P-Akt、P-mTOR、NF-κB和P-NF-κB）（流式细胞术）。用酶生酶刺激巨噬细胞，以评估吞噬活性和流式细胞术检测。结果：lqb118可降低巨噬细胞对酶酶颗粒的吞噬。这种作用可能归因于LQB抑制IL-12的产生和mTOR/NF-κB信号传导。此外，lqb118在不干扰iNOS和Akt激活的情况下降低了ROS和NO的水平。结论：这些发现显示了lqb118对巨噬细胞的抗吞噬活性，突出了该化合物作为调节巨噬细胞驱动炎症的候选化合物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunological Investigations 医学-免疫学

CiteScore

5.50

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.