Identification and bioinformatics analysis of the DUS gene in Eimeria media.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Yijin Zou, Yiyan Wang, Diyi Duan, Xun Suo, Yuanyuan Zhang
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引用次数: 0

Abstract

This study aims to explore the coding sequence (CDS) of the putative DUS gene in Eimeria media and assess its potential biological functions during the parasite's lifecycle. Initially, oocysts were isolated from fecal samples of rabbits infected with E. media, from which DNA and RNA were extracted. These extractions were used as templates for PCR to successfully amplify the CDS of the DUS gene, confirming its presence within the E. media genome. Further analysis using quantitative PCR (qPCR) demonstrated significantly higher expression of the DUS gene in the precocious line (PL) compared to the wild type (WT). This differential expression highlights a potential functional role for the DUS gene in influencing the development and sporulation processes of E. media, which may contribute to the precocious phenotype. Additionally, bioinformatics analysis provided insights into the evolutionary trends and structural characteristics of the DUS gene across different species, suggesting a broader biological significance. The elevated expression of the DUS gene in the PL suggests its critical involvement in the growth and reproductive processes of the parasite. This finding opens new avenues for research aimed at controlling E. media infection through targeted interventions in the DUS gene expression pathways.

艾美球虫DUS基因的鉴定及生物信息学分析。
本研究旨在探索艾美球虫培养基中推测的DUS基因的编码序列(CDS),并评估其在艾美球虫生命周期中的潜在生物学功能。最初,从感染了芽胞杆菌的家兔的粪便样本中分离卵囊,从中提取DNA和RNA。这些提取物被用作PCR模板,成功扩增了DUS基因的CDS,证实其存在于培养基基因组中。进一步的定量PCR (qPCR)分析表明,与野生型(WT)相比,DUS基因在早熟系(PL)中的表达明显更高。这种差异表达强调了DUS基因在影响培养基发育和产孢过程中的潜在功能作用,这可能有助于早熟表型。此外,生物信息学分析还揭示了DUS基因在不同物种间的进化趋势和结构特征,具有更广泛的生物学意义。ppl中DUS基因的高表达表明它在寄生虫的生长和繁殖过程中起着重要的作用。这一发现为通过对DUS基因表达途径的靶向干预来控制媒介弓形虫感染的研究开辟了新的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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