ATG9 promotes autophagosome formation through interaction with LC3

IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Peiqi Xu , Ting Zhang , Fangfang Yu , Lixia Guo , Yanan Yang
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引用次数: 0

Abstract

The autophagosome is a double-membrane organelle that executes macroautophagy. Previous studies have shown that the autophagosome formation is driven by autophagy-related genes, among which ATG9 is the only conserved transmembrane protein and has been shown to play a critical role in the autophagosome formation. However, how ATG9 binds to the growing autophagosome membrane has remained uncertain. Herein, we report that ATG9 binds to LC3, an essential membrane component of the autophagosome, thereby allowing ATG9 to incorporate into the autophagosome membrane. Mechanistically, we show that ATG9 interacts with LC3 through its UIM motives, which bind to the UDS site of LC3. Interrupting such UIM-UDS interaction abolishes the autophagosome association of ATG9 and suppresses the autophagosome formation. Collectively, our findings reveal a novel mechanism regulating autophagosome biogenesis and suggest that the interaction of ATG9 with LC3 is critical for ATG9 binding to the growing autophagosome membrane.
ATG9通过与LC3相互作用促进自噬体的形成。
自噬体是一种执行巨噬的双膜细胞器。以往的研究表明,自噬体的形成是由自噬相关基因驱动的,其中ATG9是唯一保守的跨膜蛋白,在自噬体的形成中起关键作用。然而,ATG9如何与生长中的自噬体膜结合仍不确定。本文中,我们报道ATG9与LC3结合,LC3是自噬体的重要膜组分,从而使ATG9结合到自噬体膜中。在机制上,我们发现ATG9通过其UIM动机与LC3相互作用,该动机结合到LC3的UDS位点。中断这种UIM-UDS相互作用可消除ATG9的自噬体关联并抑制自噬体的形成。总之,我们的研究结果揭示了一种调节自噬体生物发生的新机制,并表明ATG9与LC3的相互作用对于ATG9与生长中的自噬体膜结合至关重要。
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来源期刊
Biochemical and biophysical research communications
Biochemical and biophysical research communications 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
1400
审稿时长
14 days
期刊介绍: Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics
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