Green fabricated bimetallic zinc ferrite nanoparticles mitigate oxidative stress-induced pathogenesis

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-01-08 DOI:10.1016/j.ab.2025.115767

Shivakumar Venkataramaiah , Manjula M. Venkatappa , Rajesh Rangappa , Chikkappa Udagani , Devaraja Sannaningaiah

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Abstract

Current study evaluates the beneficial role of bio-functionalized zinc ferrite nanoparticles fabricated from an aqueous extract of Decalepis hamiltonii leaves (DHLE.ZnFe₂O₄ NPs) on sodium nitrite (NaNO₂) and Diclofenac (DFC) induced oxidative stress in RBCs and Sprague Dawley male rat models. DHLE.ZnFe₂O₄ NPs were characterized using PXRD, FTIR, SEM-EDAX, HR-TEM and VSM. The data suggests that, DHLE.ZnFe₂O₄ NPs were crystalline, ellipsoidal in shape with an average size of 10.95 nm and super paramagnetic in nature. DHLE.ZnFe₂O₄ NPs exhibited anti-oxidant properties by scavenging DPPH, H₂O₂ and reducing ferric to ferrous ions. Furthermore, DHLE.ZnFe₂O₄ NPs normalized key parameters of oxidative stress such as LPO, PCC, TT and anti-oxidant enzymes (SOD & CAT). Similar to the previous in-vitro results, DHLE.ZnFe₂O₄ NPs restored all the said stress parameters in homogenates of the liver, kidney, pancreas and heart. In addition, DHLE.ZnFe₂O₄ NPs repaired Diclofenac induced tissue damage in the liver, kidney, pancreas and heart by regulating all biochemical parameters. Most importantly, DHLE.ZnFe₂O₄ NPs exhibited anti-inflammatory, anti-diabetic, anti-thrombotic activities and were non-toxic to RBCs. In conclusion, DHLE.ZnFe₂O₄ NPs through its anti-oxidant potential ameliorate oxidative stress induced pathogenesis such as, inflammation, tissue damage, diabetes and thrombosis.

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绿色制备的双金属铁酸锌纳米颗粒减轻氧化应激诱导的发病机制。

目前的研究评估了生物功能化铁酸锌纳米颗粒的有益作用，这些纳米颗粒是由水提取物制备的。ZnFe2O4 NPs作用于亚硝酸钠（NaNO2）和双氯芬酸（DFC）诱导红细胞和Sprague Dawley雄性大鼠模型氧化应激。DHLE。采用PXRD、FTIR、SEM-EDAX、HR-TEM和VSM对ZnFe2O4纳米粒子进行了表征。数据表明，DHLE。ZnFe2O4 NPs为椭圆形结晶，平均尺寸为10.95 nm，具有超顺磁性。DHLE。ZnFe2O4 NPs通过清除DPPH、H2O2和将铁还原为亚铁离子表现出抗氧化性能。此外,DHLE。ZnFe2O4 NPs规范了氧化应激关键参数LPO、PCC、TT和抗氧化酶（SOD和cat）。与之前的体外实验结果相似，DHLE。ZnFe2O4 NPs在肝脏、肾脏、胰腺和心脏匀浆中恢复了上述所有应力参数。此外，DHLE。ZnFe2O4 NPs通过调节所有生化参数修复双氯芬酸诱导的肝、肾、胰腺和心脏组织损伤。最重要的是，DHLE。ZnFe2O4 NPs具有抗炎、抗糖尿病、抗血栓活性，对红细胞无毒。总之，DHLE。ZnFe2O4 NPs通过其抗氧化潜能改善氧化应激诱导的发病机制，如炎症、组织损伤、糖尿病和血栓形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.