Drosophila miR-263b-5p controls wing developmental growth by targeting Akt.

IF 2.5 2区 生物学 Q3 CELL BIOLOGY
Animal Cells and Systems Pub Date : 2024-12-24 eCollection Date: 2025-01-01 DOI:10.1080/19768354.2024.2444366
Chae Jeong Kim, Daegyu Jang, Do-Hwan Lim
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引用次数: 0

Abstract

Tissue growth is controlled by various signaling pathways, such as the insulin/IGF-signaling (IIS) pathway. Although IIS activation is regulated by a complex regulatory network, the mechanism underlying miRNA-based regulation of the IIS pathway in Drosophila wing development remains unclear. In this study, we found that the wing size of adult flies was negatively affected by miR-263b expression. The miR-263b-mediated alteration in wing size was linked to a reduction in wing cell number. Additionally, miR-263b overexpression in Drosophila S2 cells decreased cell proliferation and increased cell death. Consequently, we identified Akt as a direct target of miR-263b-5p and found that miR-263b-mediated wing growth regulation was due to changes in Akt expression. Co-expression of Akt in miR-263b-overexpressing wings rescued the miR-263b overexpression-mediated reduction in wing growth. These results enhance our understanding of the crucial role of miRNAs in growth regulation during Drosophila wing development.

果蝇miR-263b-5p通过靶向Akt调控翅膀发育生长。
组织生长受多种信号通路控制,如胰岛素/ igf信号通路(IIS)。尽管IIS的激活受复杂的调控网络调控,但果蝇翅膀发育中基于mirna的IIS通路调控机制尚不清楚。在本研究中,我们发现miR-263b的表达会对成年果蝇的翅膀大小产生负向影响。mir -263b介导的翅膀大小改变与翅膀细胞数量减少有关。此外,miR-263b在果蝇S2细胞中的过表达降低了细胞增殖,增加了细胞死亡。因此,我们发现Akt是miR-263b-5p的直接靶点,并发现mir -263b介导的翅膀生长调节是由于Akt表达的变化。Akt在过表达miR-263b的翅膀中共表达,挽救了miR-263b过表达介导的翅膀生长减少。这些结果增强了我们对mirna在果蝇翅膀发育过程中生长调节中的关键作用的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Cells and Systems
Animal Cells and Systems 生物-动物学
CiteScore
4.50
自引率
24.10%
发文量
33
审稿时长
6 months
期刊介绍: Animal Cells and Systems is the official journal of the Korean Society for Integrative Biology. This international, peer-reviewed journal publishes original papers that cover diverse aspects of biological sciences including Bioinformatics and Systems Biology, Developmental Biology, Evolution and Systematic Biology, Population Biology, & Animal Behaviour, Molecular and Cellular Biology, Neurobiology and Immunology, and Translational Medicine.
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