The effect of downregulation of ARL9 expression on the proliferation, metastasis and biological behavior of AGS gastric cancer cell lines.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Caihua Sun, Hongliang Yao, Jipan Liu, Shuai Wang
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引用次数: 0

Abstract

Background: Some ADP ribosylation factors (ARF) and ADP ribosylation factor-like (ARL) family are involved in the regulation of certain cancers, but the role of ADP ribosylation factor-like 9 (ARL9) in gastric tumorigenesis remains elusive.

Objectives: The main aim of this study was to evaluate the ARL9 expression within stomach cancer cells and elucidate its influence on the modulation of cancer cell behavior.

Material and methods: Differential ARL9 protein expression in normal stomach and stomach cancer tissue was ascertained through data sourced from the University of Alabama at Birmingham Cancer Data Analysis Portal (UALCAN). Quantitative analysis of ARL9 expression in gastric cancer tissue and its association with clinicopathological features was performed using quantitative polymerase chain reaction (qPCR) and western blot analysis (WB). Small interfering RNA (siRNA) was employed to suppress ARL9 protein expression in the human stomach gastric adenocarcinoma human gastric adenocarcinoma cells (AGS) cell line. Assessment of AGS gastric cancer (GC) cell proliferation, invasion and migration was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and transwell techniques.

Results: The expression of ARL9 protein exhibited a significant upregulation in GC tissue, and showed a close association between tumor dimensions (p < 0.05) and the presence of distant metastases (p < 0.05) among individuals diagnosed with GC. However, no significant link was observed with sex, age and tumor-node-metastasis (TNM) staging in gastric malignancy patients. After the introduction of si-ARL9 in the experimental set, there was a noteworthy decrease in ARL9 protein levels in AGS cells (p < 0.01). In contrast to the control cohort, the restraint of ARL9 expression significantly hampered the growth, mobility and infiltration abilities of the AGS GC cell line (p < 0.01).

Conclusions: The significant correlation of ARL9 with the biological behavior of GC indicates its potentially pivotal role in the pathophysiology of the malignancy.

下调ARL9表达对AGS胃癌细胞系增殖、转移及生物学行为的影响
背景:一些ADP核糖基化因子(ARF)和ADP核糖基化因子样(ARL)家族参与某些癌症的调控,但ADP核糖基化因子样9 (ARL9)在胃肿瘤发生中的作用尚不清楚。目的:研究胃癌细胞中ARL9的表达,并阐明其对肿瘤细胞行为调控的影响。材料和方法:通过来自阿拉巴马大学伯明翰分校癌症数据分析门户网站(UALCAN)的数据,确定正常胃和胃癌组织中ARL9蛋白的差异表达。采用定量聚合酶链反应(qPCR)和western blot分析(WB)方法定量分析胃癌组织中ARL9的表达及其与临床病理特征的关系。采用小干扰RNA (siRNA)抑制人胃腺癌细胞(AGS)细胞系中ARL9蛋白的表达。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)和transwell技术对AGS胃癌(GC)细胞的增殖、侵袭和迁移进行了评价。结果:胃癌组织中ARL9蛋白表达显著上调,且胃癌患者肿瘤大小与远处转移密切相关(p < 0.05)。然而,在胃恶性肿瘤患者中,性别、年龄和肿瘤-淋巴结-转移(TNM)分期没有明显的联系。实验组引入si-ARL9后,AGS细胞中ARL9蛋白水平明显降低(p < 0.01)。与对照组相比,ARL9表达的抑制显著抑制了AGS - GC细胞株的生长、移动性和浸润能力(p < 0.01)。结论:ARL9与胃癌生物学行为显著相关,提示其在胃癌病理生理中具有潜在的关键作用。
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来源期刊
Advances in Clinical and Experimental Medicine
Advances in Clinical and Experimental Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
3.70
自引率
4.80%
发文量
153
审稿时长
6-12 weeks
期刊介绍: Advances in Clinical and Experimental Medicine has been published by the Wroclaw Medical University since 1992. Establishing the medical journal was the idea of Prof. Bogumił Halawa, Chair of the Department of Cardiology, and was fully supported by the Rector of Wroclaw Medical University, Prof. Zbigniew Knapik. Prof. Halawa was also the first editor-in-chief, between 1992-1997. The journal, then entitled "Postępy Medycyny Klinicznej i Doświadczalnej", appeared quarterly. Prof. Leszek Paradowski was editor-in-chief from 1997-1999. In 1998 he initiated alterations in the profile and cover design of the journal which were accepted by the Editorial Board. The title was changed to Advances in Clinical and Experimental Medicine. Articles in English were welcomed. A number of outstanding representatives of medical science from Poland and abroad were invited to participate in the newly established International Editorial Staff. Prof. Antonina Harłozińska-Szmyrka was editor-in-chief in years 2000-2005, in years 2006-2007 once again prof. Leszek Paradowski and prof. Maria Podolak-Dawidziak was editor-in-chief in years 2008-2016. Since 2017 the editor-in chief is prof. Maciej Bagłaj. Since July 2005, original papers have been published only in English. Case reports are no longer accepted. The manuscripts are reviewed by two independent reviewers and a statistical reviewer, and English texts are proofread by a native speaker. The journal has been indexed in several databases: Scopus, Ulrich’sTM International Periodicals Directory, Index Copernicus and since 2007 in Thomson Reuters databases: Science Citation Index Expanded i Journal Citation Reports/Science Edition. In 2010 the journal obtained Impact Factor which is now 1.179 pts. Articles published in the journal are worth 15 points among Polish journals according to the Polish Committee for Scientific Research and 169.43 points according to the Index Copernicus. Since November 7, 2012, Advances in Clinical and Experimental Medicine has been indexed and included in National Library of Medicine’s MEDLINE database. English abstracts printed in the journal are included and searchable using PubMed http://www.ncbi.nlm.nih.gov/pubmed.
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