Role of Mutyh in Oxidative Stress Damage in Retinopathy of Prematurity.

Q4 Medicine
Hui-Juan Li, Jie Tang, Rui Cheng
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引用次数: 0

Abstract

Objective To explore the role of the base mismatch repair gene Mutyh in retinopathy of prematurity(ROP). Methods Mutyh(-/-)and wild-type(WT)mice were used for the modeling of oxygen-induced retinopathy.The retinal oxidative stress was examined,and the ultrastructures of photoreceptors and mitochondria were observed.The biomarkers of photoreceptors and mitochondria were tested.Furthermore,the photoreceptor cell line 661W was treated with hydrogen peroxide for the modeling of oxidative stress.In the cell model,and the oxidative stress and photoreceptor functions in the cells were measured. Results In both the mouse and cell models,the expression of Mutyh was up-regulated.Mutyh knockout in mice and knockdown in cells exerted negative effects on photoreceptors and mitochondria.Mutyh overexpression showed protective functions in the cell model,indicating that Mutyh played a role in repairing photoreceptors and mitochondria. Conclusions Mutyh showed the potential to become a biomarker of ROP.Increasing Mutyh expression might have therapeutic effects on ROP,which needs further validation.

Mutyh在早产儿视网膜病变氧化应激损伤中的作用。
目的探讨碱基错配修复基因Mutyh在早产儿视网膜病变(ROP)中的作用。方法采用Mutyh(-/-)和野生型(WT)小鼠建立氧致视网膜病变模型。观察视网膜氧化应激,光感受器和线粒体超微结构。检测光感受器和线粒体的生物标志物。此外,用过氧化氢处理光感受器细胞系661W以模拟氧化应激。在细胞模型中,测定细胞的氧化应激和感光功能。结果在小鼠和细胞模型中,Mutyh的表达均上调。小鼠的Mutyh敲除和细胞的敲除对光感受器和线粒体产生负面影响。在细胞模型中,Mutyh过表达显示出保护功能,表明Mutyh在修复光感受器和线粒体中发挥作用。结论muyh具有成为ROP生物标志物的潜力。增加Mutyh表达可能对ROP有治疗作用,有待进一步验证。
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来源期刊
中国医学科学院学报
中国医学科学院学报 Medicine-Medicine (all)
CiteScore
0.60
自引率
0.00%
发文量
6813
期刊介绍: Acta Academiae Medicinae Sinicae was founded in February 1979. It is a comprehensive medical academic journal published in China and abroad, supervised by the Ministry of Health of the People's Republic of China and sponsored by the Chinese Academy of Medical Sciences and Peking Union Medical College. The journal mainly reports the latest research results, work progress and dynamics in the fields of basic medicine, clinical medicine, pharmacy, preventive medicine, biomedicine, medical teaching and research, aiming to promote the exchange of medical information and improve the academic level of medicine. At present, the journal has been included in 10 famous foreign retrieval systems and their databases [Medline (PubMed online version), Elsevier, EMBASE, CA, WPRIM, ExtraMED, IC, JST, UPD and EBSCO-ASP]; and has been included in important domestic retrieval systems and databases [China Science Citation Database (Documentation and Information Center of the Chinese Academy of Sciences), China Core Journals Overview (Peking University Library), China Science and Technology Paper Statistical Source Database (China Science and Technology Core Journals) (China Institute of Scientific and Technological Information), China Science and Technology Journal Paper and Citation Database (China Institute of Scientific and Technological Information)].
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