Retention mechanism on phosphodiester stationary phases in HILIC and purely aqueous mobile phase, Part I: The problem of hold-up volume determination.

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2025-02-08 Epub Date: 2024-12-28 DOI:10.1016/j.chroma.2024.465627

Ivett Bacskay, Alessandro Buratti, Martina Catani, Mikołaj Dembek, Szymon Bocian, Bogusław Buszewski, Attila Felinger

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引用次数: 0

Abstract

Non-destructive chromatographic methods were used to determine the hold-up volumes of four self-packed columns containing embedded phosphate groups. The stationary phases are named Diol-P-C10, Diol-P-C18, Diol-P-Benzyl and Diol-P-Chol. The hydrophobicity of organic ligands bound to the phosphate group increases in the benzyl< decyl < octadecyl

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磷酸二酯固定相在HILIC和纯水流动相中的保留机理，第一部分：保留体积的测定问题。

采用非破坏性色谱法测定含有包埋磷酸基团的四根自填充柱的持有体积。固定相分别命名为：二醇- p - c10、二醇- p - c18、二醇- p -苄基和二醇- p - chol。与磷酸基团结合的有机配体的疏水性在苯基<癸基<十八基中增加

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.