Exploring Multiplex Immunohistochemistry (mIHC) Techniques and Histopathology Image Analysis: Current Practice and Potential for Clinical Incorporation

IF 2.9 2区医学 Q2 ONCOLOGY

Cancer Medicine Pub Date : 2025-01-07 DOI:10.1002/cam4.70523

Aria Kaiyuan Sun, Song Fan, Siu Wai Choi

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引用次数: 0

Abstract

Background

By simultaneously staining multiple immunomarkers on a single tissue section, multiplexed immunohistochemistry (mIHC) enhances the amount of information that can be observed in a single tissue section and thus can be a powerful tool to visualise cellular interactions directly in the tumour microenvironment. Performing mIHC remains technically and practically challenging, and this technique has many limitations if not properly validated. However, with proper validation, heterogeneity between histopathological images can be avoided.

Aims

This review aimed to summarize the currently used methods and to propose a standardised method for effective mIHC.

Materials and Methods

An extensive literature review was conducted to identify different methods currently in use for mIHC.

Results

Guidelines for antibody selection, panel design, antibody validation and analytical strategies are given. The advantages and disadvantages of each method are discussed.

Conclusion

This review summarizes widely used pathology imaging software and discusses the potential for automation of pathology image analysis so that mIHC technology can be a truly powerful tool for research as well as clinical use.

查看原文本刊更多论文

探索多重免疫组织化学（mIHC）技术和组织病理学图像分析：目前的实践和临床应用的潜力。

背景：通过在单个组织切片上同时染色多个免疫标记物，多重免疫组织化学（mIHC）增强了在单个组织切片中可以观察到的信息量，因此可以成为直观观察肿瘤微环境中细胞相互作用的有力工具。执行mIHC在技术和实践上仍然具有挑战性，如果不适当验证，该技术有许多局限性。然而，通过适当的验证，可以避免组织病理图像之间的异质性。目的：本综述旨在总结目前使用的方法，并提出一种标准化的有效的mIHC方法。材料和方法：进行了广泛的文献综述，以确定目前用于mIHC的不同方法。结果：给出了抗体选择、面板设计、抗体验证和分析策略的指南。讨论了每种方法的优缺点。结论：本文综述了广泛使用的病理成像软件，并讨论了病理图像分析自动化的潜力，从而使mIHC技术成为研究和临床应用的真正强大工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Medicine ONCOLOGY-

CiteScore

5.50

自引率

2.50%

发文量

907

审稿时长

19 weeks

期刊介绍： Cancer Medicine is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research from global biomedical researchers across the cancer sciences. The journal will consider submissions from all oncologic specialties, including, but not limited to, the following areas: Clinical Cancer Research Translational research ∙ clinical trials ∙ chemotherapy ∙ radiation therapy ∙ surgical therapy ∙ clinical observations ∙ clinical guidelines ∙ genetic consultation ∙ ethical considerations Cancer Biology: Molecular biology ∙ cellular biology ∙ molecular genetics ∙ genomics ∙ immunology ∙ epigenetics ∙ metabolic studies ∙ proteomics ∙ cytopathology ∙ carcinogenesis ∙ drug discovery and delivery. Cancer Prevention: Behavioral science ∙ psychosocial studies ∙ screening ∙ nutrition ∙ epidemiology and prevention ∙ community outreach. Bioinformatics: Gene expressions profiles ∙ gene regulation networks ∙ genome bioinformatics ∙ pathwayanalysis ∙ prognostic biomarkers. Cancer Medicine publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.