Droplets in open microfluidics: generation, manipulation, and application in cell analysis

IF 6.1 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2025-01-08 DOI:10.1039/D4LC00646A
Jiaxu Lin, Ying Hou, Qiang Zhang and Jin-Ming Lin
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引用次数: 0

Abstract

Open droplet microfluidics is an emerging technology that generates, manipulates, and analyzes droplets in open configuration systems. Droplets function as miniaturized reactors for high-throughput analysis due to their compartmentalization and parallelization, while openness enables addressing and accessing the targeted contents. The convergence of two technologies facilitates the localization and intricate manipulation of droplets using external tools, showing great potential in large-scale chemical and biological applications, particularly in cell analysis. In this review, we first introduce various methods of droplet generation and manipulation in open environments. Next, we summarize the typical applications of open droplet systems in cell culture. Then, a comprehensive overview of cell analysis is provided, including nucleic acids, proteins, metabolites, and behaviors. Finally, we present a discussion of current challenges and perspectives in this field.

Abstract Image

开放微流体中的液滴:产生、操作和在细胞分析中的应用。
开放液滴微流体是一种新兴的技术,在开放配置系统中产生,操纵和分析液滴。液滴作为高通量分析的小型反应器,由于它们的分区化和并行化,而开放性使寻址和访问目标内容成为可能。这两种技术的融合促进了液滴的定位和使用外部工具的复杂操作,在大规模化学和生物应用中显示出巨大的潜力,特别是在细胞分析中。在这篇综述中,我们首先介绍了在开放环境中产生和操纵液滴的各种方法。接下来,我们总结了开放液滴系统在细胞培养中的典型应用。然后,提供了细胞分析的全面概述,包括核酸,蛋白质,代谢物和行为。最后,我们对该领域当前的挑战和前景进行了讨论。
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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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