In Situ Ag-Seeded Lamellar Ti3C2 Nanosheets: An Electroactive Interface for Noninvasive Diagnosis of Oral Carcinoma via Salivary TNF-α Sensing.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2025-01-20 Epub Date: 2025-01-09 DOI:10.1021/acsabm.4c01379
Sunil Vasu, Damini Verma, Omal Surya Souraph S, Katha Anki Reddy, Gopinath Packirisamy, Uday Kumar S
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引用次数: 0

Abstract

In the fast-paced quest for early cancer detection, noninvasive screening techniques have emerged as game-changers, offering simple and accessible avenues for precession diagnostics. In line with this, our study highlights the potential of silver nanoparticle-decorated titanium carbide MXene nanosheets (Ti3C2_AgNPs) as an electroactive interface for the noninvasive diagnosis of oral carcinoma based on the prevalence of the salivary biomarker, tumor necrosis factor-α (TNF-α). An in situ reduction was utilized to synthesize the Ti3C2_AgNPs nanohybrid, wherein Ti3C2 acts as the reducing agent, and the resulting nanohybrid was subjected to various characterization techniques to examine the optical, structural, and morphological attributes. The results revealed that spherical AgNPs formed on the surface of Ti3C2 MXene nanosheets by virtue of the low-valent Ti species present in Ti3C2, which facilitated the reduction of AgNO3 to AgNPs. Furthermore, the electrochemical characterization of the nanohybrid-modified screen-printed electrode (Ti3C2_AgNPs/SPE) indicated enhanced heterogeneous electron transfer kinetics. With these encouraging results, the Ti3C2_AgNPs nanohybrid was employed as an immobilization matrix for TNF-α antibodies and applied for electrochemical sensing. Analytical studies of the fabricated immunosensor, conducted by differential pulse voltammetry (DPV), exhibited a broader linear range (1 to 180 pg mL-1), a low limit of detection (0.97 pg mL-1), and high sensitivity (1.214 μA mL pg-1 cm-2) and specificity, even in artificial saliva, indicating its reliability for oral carcinoma diagnosis. Therefore, the Ti3C2_AgNP nanohybrid seems a promising candidate for the effective sensing of TNF-α and could also be explored for other biomarkers.

原位ag种子层状Ti3C2纳米片:通过唾液TNF-α传感无创诊断口腔癌的电活性界面。
在对早期癌症检测的快速探索中,非侵入性筛查技术已经成为游戏规则的改变者,为岁差诊断提供了简单而容易获得的途径。基于此,我们的研究强调了银纳米颗粒装饰碳化钛MXene纳米片(Ti3C2_AgNPs)作为基于唾液生物标志物肿瘤坏死因子-α (TNF-α)患病率的口腔癌无创伤诊断的电活性界面的潜力。以Ti3C2为还原剂,采用原位还原法制备了Ti3C2_AgNPs纳米杂化物,并对所制备的纳米杂化物进行了光学、结构和形态表征。结果表明,在Ti3C2 MXene纳米片表面形成球形AgNPs是由于Ti3C2中存在的低价Ti物质,这有利于AgNO3还原成AgNPs。此外,纳米杂化修饰的丝网印刷电极(Ti3C2_AgNPs/SPE)的电化学表征表明,其非均相电子转移动力学得到了增强。基于这些令人鼓舞的结果,Ti3C2_AgNPs纳米杂化物被用作TNF-α抗体的固定基质,并应用于电化学传感。利用差分脉冲伏安法(DPV)对制备的免疫传感器进行分析研究,显示出较宽的线性范围(1 ~ 180 pg mL-1),低检测限(0.97 pg mL-1),高灵敏度(1.214 μA mL pg-1 cm-2)和特异性,甚至在人工唾液中也能检测到,表明其用于口腔癌诊断的可靠性。因此,Ti3C2_AgNP纳米杂化物似乎是有效检测TNF-α的有希望的候选者,也可以用于其他生物标志物的探索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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