Clinically Deployable Electro-Immunosensing Device Comprising Bioactive Glass-MWCNT for Alkaline Phosphatase Detection in Human Serum Samples.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-01-20 Epub Date: 2025-01-09 DOI:10.1021/acsabm.4c01569

Darshna, Indrani Nandi, Pradeep Srivastava, Pranjal Chandra

{"title":"Clinically Deployable Electro-Immunosensing Device Comprising Bioactive Glass-MWCNT for Alkaline Phosphatase Detection in Human Serum Samples.","authors":"Darshna, Indrani Nandi, Pradeep Srivastava, Pranjal Chandra","doi":"10.1021/acsabm.4c01569","DOIUrl":null,"url":null,"abstract":"Bone is a dynamic tissue that serves several purposes in the human body, including storing calcium, forming blood cells, and protecting and supporting the body's organs. Alkaline phosphatase (ALP) is secreted into the circulation by osteoblasts, the cells responsible for making bone. It attaches to the surface of osteoblast cells or matrix vesicles. Accordingly, ALP is present in serum and is a popular biomarker for the evaluation of bone disease and other disorders in clinical evaluations. In this study, a composite probe made of bioactive glass (BG) and multiwalled carbon nanotubes (MWCNT) was used to detect ALP through electrochemical impedance spectroscopy (EIS) without the need for labels. By combination of physical methods with electrochemical analysis, the biosensor probe was characterized. The analytical performance of the biosensor was evaluated using EIS, and the results revealed that it has a limit of detection (LOD) of 2.29 ± 0.35 U/L and a linear dynamic range (LDR) of 25-600 U/L; both of which are within the acceptable range for ALP detection in clinics. When tested against serum interfering chemicals, the biosensor probe that was designed shows a strong selectivity for ALP (Ksel < 0.06). In addition, human serum and fetal bovine serum were used to test the system's ability to detect ALP in biological samples.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"741-753"},"PeriodicalIF":4.6000,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/acsabm.4c01569","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/9 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Bone is a dynamic tissue that serves several purposes in the human body, including storing calcium, forming blood cells, and protecting and supporting the body's organs. Alkaline phosphatase (ALP) is secreted into the circulation by osteoblasts, the cells responsible for making bone. It attaches to the surface of osteoblast cells or matrix vesicles. Accordingly, ALP is present in serum and is a popular biomarker for the evaluation of bone disease and other disorders in clinical evaluations. In this study, a composite probe made of bioactive glass (BG) and multiwalled carbon nanotubes (MWCNT) was used to detect ALP through electrochemical impedance spectroscopy (EIS) without the need for labels. By combination of physical methods with electrochemical analysis, the biosensor probe was characterized. The analytical performance of the biosensor was evaluated using EIS, and the results revealed that it has a limit of detection (LOD) of 2.29 ± 0.35 U/L and a linear dynamic range (LDR) of 25-600 U/L; both of which are within the acceptable range for ALP detection in clinics. When tested against serum interfering chemicals, the biosensor probe that was designed shows a strong selectivity for ALP (K_sel < 0.06). In addition, human serum and fetal bovine serum were used to test the system's ability to detect ALP in biological samples.

查看原文本刊更多论文

临床可部署的电免疫传感装置，包括生物活性玻璃- mwcnt，用于检测人血清样品中的碱性磷酸酶。

骨骼是一种动态组织，在人体中有多种用途，包括储存钙，形成血细胞，保护和支持身体的器官。碱性磷酸酶（ALP）由成骨细胞分泌到血液循环中，成骨细胞负责制造骨骼。它附着在成骨细胞或基质囊泡的表面。因此，ALP存在于血清中，是一种流行的生物标志物，用于评估骨病和其他临床评估疾病。本研究利用生物活性玻璃（BG）和多壁碳纳米管（MWCNT）组成的复合探针，通过电化学阻抗谱（EIS）检测ALP，无需标记。采用物理方法和电化学分析相结合的方法，对生物传感器探针进行了表征。结果表明，该传感器的检测限（LOD）为2.29±0.35 U/L，线性动态范围（LDR）为25 ~ 600 U/L；两者均在临床ALP检测可接受范围内。在对血清干扰物的检测中，所设计的生物传感器探针对ALP具有较强的选择性（Ksel < 0.06）。此外，还使用人血清和胎牛血清来测试该系统检测生物样品中ALP的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.