Magnetic LDH Coated with DOX and CUR Physically Co-loaded onto PEG for Targeted and Controlled Co-delivery of Drugs to Liver Cancer Cells

IF 4.7 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2024-10-10 DOI:10.1007/s10924-024-03430-2

Soheyla Karimi, Hassan Namazi

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引用次数: 0

Abstract

Nowadays, pH-sensitive and controlled drug delivery systems are urgently required in targeted cancer therapy to increase the drug efficacy and reduce the side effects of cytotoxic drugs. Hence, the present research describes the preparation of Mg-Al-Ca layered double hydroxide (LDH@Fe₃O₄) coated with polyethylene glycol (PEG)/doxorubicin (DOX)/curcumin (CUR) for targeted and controlled delivery of DOX and CUR (LDH@Fe₃O₄-PEG-DOX-CUR) to liver cancer treatment. The prepared nanocarriers were characterized by FT-IR, FE-SEM, EDX, XRD, BET, VSM, and Zeta potential. The in vitro release investigations of DOX and CUR from the PEG-DOX-CUR and LDH@Fe₃O₄-PEG-DOX-CUR confirmed a pH-dependent behavior. The release of drugs from these systems follows the Korsmeyer-Peppas kinetics model. In addition, the LDH@Fe₃O₄ demonstrated low cytotoxicity and good biocompatibility, as confirmed by in vitro cytotoxicity and DAPI tests against L929 (non-cancerous cells) and HepG2 (human liver cancer cells) cell lines. Whereas, the PEG-DOX-CUR and LDH@Fe₃O₄-PEG-DOX-CUR showed higher cytotoxicity effects against HepG2 cells due to targeted co-delivery of DOX and CUR. Based on the findings, the developed nanocarriers have the potential to be utilized as targeted co-drug delivery systems in biomedical applications.

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磁性LDH包被DOX和CUR物理上共负载到PEG上，用于靶向和控制药物共同递送到肝癌细胞

目前，肿瘤靶向治疗迫切需要ph敏感和可控的给药系统，以提高药物疗效，减少细胞毒性药物的副作用。因此，本研究描述了制备聚乙二醇(PEG)/阿霉素(DOX)/姜黄素（CUR）包裹的Mg-Al-Ca层状双氢氧化物（LDH@Fe3O4），用于靶向和控制地递送DOX和CUR （LDH@Fe3O4-PEG-DOX-CUR）以治疗肝癌。采用FT-IR、FE-SEM、EDX、XRD、BET、VSM和Zeta电位对所制备的纳米载体进行了表征。DOX和CUR从PEG-DOX-CUR和LDH@Fe3O4-PEG-DOX-CUR的体外释放研究证实了ph依赖性行为。从这些系统中释放药物遵循Korsmeyer-Peppas动力学模型。此外，通过对L929（非癌细胞）和HepG2（人肝癌细胞）细胞系的体外细胞毒性和DAPI试验证实，LDH@Fe3O4具有低细胞毒性和良好的生物相容性。然而，由于DOX和CUR的靶向共递送，PEG-DOX-CUR和LDH@Fe3O4-PEG-DOX-CUR对HepG2细胞表现出更高的细胞毒性作用。基于这些发现，所开发的纳米载体具有在生物医学应用中作为靶向共递送系统的潜力。

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.