Ultra-facile synthesis of CuO nanoclusters with excellent antibacterial activity and their antimicrobial mechanism study

IF 2.3 4区材料科学 Q2 MATERIALS SCIENCE, CERAMICS

Journal of Sol-Gel Science and Technology Pub Date : 2024-10-22 DOI:10.1007/s10971-024-06569-5

Guoqiang Yang, Ye Hong, Yimei Wang, Xuechao Shi, Shuxian Hou, Xinxin Liu, Yuxuan Wang, Fei Ge, Jun Wang

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引用次数: 0

Abstract

It is well known that bacterial infections pose a great threat to human health and life, and the situation has intensified because of the emergence of drug-resistant bacteria, especially in the medical field, the number of deaths due to super-bacteria infections that result from antibiotic misuse continues to increase every year. Researchers have been working hard and trying to find a suitable method to fight against bacterial infections, however, there is still no effective method to fight drug-resistant bacterial infections. In this study, an ultra-facile strategy was built to synthesize Copper Oxide Nanoclusters (CuO NCs), that exhibited broad-spectrum bactericidal activity against common pathogenic Gram-positive and Gram-negative bacteria. The synthesized CuO NCs exhibited a cluster structure with good stability and biocompatibility. The antibacterial mechanism revealed that the synthesized CuO NCs can cause bacterial death in multiple ways, including disrupting the bacterial cell membrane and ablating the biofilm, inducing the generation of ROS, and leading to nucleic acid leakage of bacteria. CuO NCs are simple to synthesize, have strong antibacterial activity, and are expected to open new avenues of antibacterial activity in the severe antibiotic environment due to the combination of antibacterial mechanisms that make it difficult for bacteria to develop drug resistance quickly.

Graphical Abstract

In this study, we designed and synthesized copper oxide nanoclusters (CuO NCs) with favorable biosafety and stability for combating bacterial infection problems. The material possesses several advantages that including: (1) CuO NCs are synthesized in a straightforward and expeditious method. (2) CuO NCs have been demonstrated to possess good biocompatibility. (3) The synthesized CuO NCs exhibit broad-spectrum antimicrobial activity against E. coli and S. aureus, as well as for their drug-resistant strains. (4) The synthesized CuO NCs are capable of effectively removing biofilms formed by bacteria and promoting ROS generation, which disrupts the bacterial cell membrane, induces nucleic acid leakage, and ultimately cause the death of bacteria. This study employing an efficient strategy to synthesize CuO NCs with excellent broad-spectrum anti-bacterial ability, offering a promising method for addressing the growing challenge of multi-drug resistant bacteria.

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具有优异抗菌活性的氧化铜纳米团簇的超简便合成及其抗菌机理研究

众所周知，细菌感染对人类健康和生命构成极大威胁，由于耐药细菌的出现，这种情况更加严重，特别是在医疗领域，由于滥用抗生素导致的超级细菌感染造成的死亡人数每年都在增加。研究人员一直在努力寻找一种合适的方法来对抗细菌感染，然而，仍然没有有效的方法来对抗耐药细菌感染。在这项研究中，建立了一种超简单的策略来合成氧化铜纳米簇（CuO NCs），该纳米簇对常见的致病性革兰氏阳性和革兰氏阴性细菌具有广谱杀菌活性。合成的纳米CuO具有良好的簇状结构，具有良好的稳定性和生物相容性。抑菌机制揭示，合成的CuO NCs可通过破坏细菌细胞膜和吞噬生物膜、诱导ROS生成、导致细菌核酸泄漏等多种途径导致细菌死亡。CuO NCs合成简单，抑菌活性强，结合多种抑菌机制使细菌难以快速产生耐药性，有望在严峻的抗生素环境中开辟抑菌活性的新途径。在本研究中，我们设计并合成了具有良好生物安全性和稳定性的氧化铜纳米团簇（CuO NCs），用于对抗细菌感染问题。该材料具有以下优点：(1)合成CuO纳米碳化物的方法简单、快捷。(2) CuO NCs具有良好的生物相容性。(3)合成的CuO NCs对大肠杆菌和金黄色葡萄球菌及其耐药菌株具有广谱抗菌活性。(4)合成的CuO NCs能够有效去除细菌形成的生物膜，促进ROS生成，破坏细菌细胞膜，诱发核酸渗漏，最终导致细菌死亡。本研究采用一种高效的策略合成了具有优异广谱抗菌能力的CuO NCs，为解决多重耐药菌日益增长的挑战提供了一种有希望的方法。

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来源期刊

Journal of Sol-Gel Science and Technology 工程技术-材料科学：硅酸盐

CiteScore

4.70

自引率

4.00%

发文量

280

审稿时长

2.1 months

期刊介绍： The primary objective of the Journal of Sol-Gel Science and Technology (JSST), the official journal of the International Sol-Gel Society, is to provide an international forum for the dissemination of scientific, technological, and general knowledge about materials processed by chemical nanotechnologies known as the "sol-gel" process. The materials of interest include gels, gel-derived glasses, ceramics in form of nano- and micro-powders, bulk, fibres, thin films and coatings as well as more recent materials such as hybrid organic-inorganic materials and composites. Such materials exhibit a wide range of optical, electronic, magnetic, chemical, environmental, and biomedical properties and functionalities. Methods for producing sol-gel-derived materials and the industrial uses of these materials are also of great interest.