A Highly Sensitive Test System for Measuring the Phenolic Index of Wastewater Based on a Biocompatible Composite Material with Carbon Nanotubes

IF 4.7 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2024-10-04 DOI:10.1007/s10924-024-03421-3

Tatyana Lavrova, Anna Kharkova, Roman Perchikov, Maria Gertsen, Andrey Shadrin, Vyacheslav Arlyapov

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Abstract

A biosensor for the determination of the phenol index in water bodies has been developed using enzymes immobilized on the redox-active polymer surface of a graphite paste electrode. Tyrosinase, laccase, and bacterial cell membrane fractions were used as biological materials. Redox polymers based on bovine serum albumin (BSA) and various electron transport mediators were synthesized for immobilization. It has been shown that a redox polymer based on ferrocene (FC) combined with single-walled carbon nanotubes (SWCNTs) is more promising for use in biosensors than a polymer based on safranin (SAF) based on electrochemical and analytical parameters. The best results were obtained with the “BSA-FC-SWCNT-tyrosinase” bioanalytical system, which has a lower limit of detectable concentrations of 1 × 10⁻³ mg/L, indicating the possibility of monitoring water environments with a phenol concentration corresponding to the maximum permissible concentration (MPC). The maximum analytical signal was generated at a temperature of 30 °С and a pH of 6.8. The biosensor was stable at concentrations of heavy metal ions up to 100 times the MPC, as well as at NaCl concentrations up to 5%. The system was tested on river water samples containing phenol. A statistical analysis of the results showed no significant difference between the biosensor results and those of standard analytical methods. The proposed approach will significantly reduce the analysis time, its labor intensity and cost.

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基于碳纳米管生物相容性复合材料的高灵敏度废水酚指数测试系统

将酶固定在石墨糊电极的氧化还原活性聚合物表面，研制了一种测定水体中苯酚指数的生物传感器。以酪氨酸酶、漆酶和细菌细胞膜组分作为生物材料。合成了基于牛血清白蛋白（BSA）和多种电子传递介质的氧化还原聚合物用于固定化。研究表明，基于二茂铁（FC）结合单壁碳纳米管（SWCNTs）的氧化还原聚合物比基于红花素（SAF）的基于电化学和分析参数的聚合物更有希望用于生物传感器。“bsa - fc - swcnt -酪氨酸酶”生物分析系统的检测结果最好，其检测浓度下限为1 × 10⁻³mg/L，表明用最大允许浓度（MPC）对应的苯酚浓度监测水环境是可能的。在温度为30°С和pH为6.8时产生最大的分析信号。该生物传感器在高达100倍MPC的重金属离子浓度和高达5%的NaCl浓度下都是稳定的。该系统在含酚的河水样品中进行了测试。统计分析结果表明，生物传感器的结果与标准分析方法的结果无显著差异。该方法将大大减少分析时间、劳动强度和成本。

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.