Periodontitis Increases Gingival, Serum and Hippocampus β‐Amyloid Expressions but Reduces Neurovascular Coupling in Basilar Artery of Rats

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology Pub Date : 2025-01-09 DOI:10.1111/jcpe.14110

Earl Fu, Kuo‐Feng Huang, Hsi‐Hsien Chang, Hui‐Hwa Tseng, Stephen Shei‐Dei Yang

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引用次数: 0

Abstract

AimNeurodegenerative diseases are characterized by early increased beta‐amyloid (Aβ) and decreased cerebrovascular reactivity. We investigated Aβ in gingiva, serum or hippocampus and neurovascular reactivity in basilar artery (BA) of periodontitis rats, to test the impact of Aβ on BA vasoreactivity ex vivo.Materials and MethodsPeriodontitis was induced in 32 rats using silk‐ligation. Rats were sacrificed at weeks 0, 1, 2 and 4. Gingival TNF‐α, IL‐1β and Aβ were quantified via immunoblotting. Alveolar bone destruction was examined by micro‐computed tomography. Serum and hippocampus Aβ values were measured by enzyme‐linked immunosorbent assay and fluorescence staining, respectively. Vasoreactivity was measured by myography on isolated BA.ResultsFrom Week 1, gingival TNF‐α and IL‐1β and bone destruction increased. Gingiva, serum and hippocampus Aβ values increased from Week 2. Nicotine‐induced BA relaxation declined from Week 2, while acetylcholine‐induced relaxation decreased by Week 4. Bone loss correlated with Aβ and nicotine‐induced relaxation. Correlations were observed between Aβs in tissues, between two induced BA relaxations and between Aβ expressions and the induced relaxations. Ex vivo, Aβ reduced nicotine‐ and isoproterenol‐induced relaxations but not electrical stimulated relaxation.ConclusionsPeriodontitis may increase Aβ expressions and reduce BA neurovascular reactivity, with Aβ contributing to this abnormal neurovascular coupling.

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牙周炎增加大鼠牙龈、血清和海马β -淀粉样蛋白表达，但减少基底动脉神经血管偶联

神经退行性疾病的特征是早期β -淀粉样蛋白（Aβ）升高和脑血管反应性降低。通过对牙周炎大鼠牙龈、血清、海马中Aβ水平和基底动脉（BA）神经血管反应性的研究，探讨Aβ对基底动脉（BA）血管反应性的影响。材料与方法采用丝线结扎法诱导32只大鼠患牙周炎。大鼠于第0、1、2、4周处死。免疫印迹法定量检测牙龈TNF - α、IL - 1β和Aβ。通过微计算机断层扫描检查牙槽骨破坏情况。采用酶联免疫吸附法和荧光染色法分别测定血清和海马Aβ值。用离体BA肌图测定血管反应性。结果从第1周开始，牙龈TNF - α、IL - 1β和骨破坏增加。牙龈、血清和海马Aβ值从第2周开始升高。尼古丁诱导的BA松弛从第2周开始下降，而乙酰胆碱诱导的BA松弛从第4周开始下降。骨质流失与Aβ和尼古丁诱导的松弛相关。组织中Aβs、诱导的两种BA松弛之间以及Aβ表达与诱导的松弛之间存在相关性。体外实验中，Aβ能减少尼古丁和异丙肾上腺素诱导的松弛，但不能减少电刺激的松弛。结论牙周炎可能导致Aβ表达升高，BA神经血管反应性降低，Aβ参与了这种异常的神经血管偶联。

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来源期刊

Journal of Clinical Periodontology 医学-牙科与口腔外科

CiteScore

13.30

自引率

10.40%

发文量

175

审稿时长

3-8 weeks

期刊介绍： Journal of Clinical Periodontology was founded by the British, Dutch, French, German, Scandinavian, and Swiss Societies of Periodontology. The aim of the Journal of Clinical Periodontology is to provide the platform for exchange of scientific and clinical progress in the field of Periodontology and allied disciplines, and to do so at the highest possible level. The Journal also aims to facilitate the application of new scientific knowledge to the daily practice of the concerned disciplines and addresses both practicing clinicians and academics. The Journal is the official publication of the European Federation of Periodontology but wishes to retain its international scope. The Journal publishes original contributions of high scientific merit in the fields of periodontology and implant dentistry. Its scope encompasses the physiology and pathology of the periodontium, the tissue integration of dental implants, the biology and the modulation of periodontal and alveolar bone healing and regeneration, diagnosis, epidemiology, prevention and therapy of periodontal disease, the clinical aspects of tooth replacement with dental implants, and the comprehensive rehabilitation of the periodontal patient. Review articles by experts on new developments in basic and applied periodontal science and associated dental disciplines, advances in periodontal or implant techniques and procedures, and case reports which illustrate important new information are also welcome.