Anxiety of microbially synthesized Fe3O4-SPIONs on embryonic/larval ontogeny in red tilapia (Oreochromis sp.)

Abstract

Iron oxide nanoparticles, recognized for their superparamagnetic properties, are promising for future healthcare therapies. However, their extensive use in medicine and electronics contributes to their discharge into our environments, highlighting the need for further research on their cellular damage effects on aquatic organisms. While the detrimental properties of other compounds have been stated in the early-life stages of fish, the cytotoxic consequences of superparamagnetic iron oxide nanoparticles (SPIONs) in these stages are still unexplored. Therefore, using the red tilapia (Oreochromis sp.) as a model organism, this study is the first to talk about the subtle cellular alterations caused by biologically induced biomineralized Fe₃O₄-SPIONs by Bacillus sp. in the early-life stages. Once the red tilapia eggs were fertilized, they were challenged to different doses of SPIONs (0, 5, 10, 15, and 30 mg/l), and their tenfold increases (50, 100, 150, and 300 mg/l) for 72 h. The hatching rate, malformation rate, body length, and deformities of the larvae were all studied. Our research showed that iron oxide nanoparticles were harmful to the early stages of life in red tilapia embryos and larvae. They slowed hatching delay, a decrease in survival rate, an increase in heart rate, bleeding, arrested development, and membrane damage and changed the axis’s physiological structure. Additionally, results indicated numerous deformities of red tilapia larvae, with lordosis, kyphosis, and scoliosis once subjected to 50 and 150 mg/l of SPIONs concentrations, respectively. This study could assist us in recognizing the risk and evaluating the disrupting potential of nanoparticles. The key objective of this inquiry is to describe the existing features of the produced magnetite SPIONs (29.44 g/l) including their morphological, chemical, and magnetic characteristics. Illustrate their current role in medicinal applications and aquatic organisms by studying in vivo cytotoxic effects to motivate the development of enhanced SPIONs systems. As a recommendation, more research is needed to completely understand how various exposure endpoints of SPIONs disturb the bodies of red tilapia in the early stages.

• Biogenic SPIONs: a material of the future.

• Characterization is essential to assess the functional properties of the produced SPIONs.

• Fe₃O₄-SPIONs’ impact on the red tilapia ontogeny.