Reproductive and detoxifying responses of Panonychus citri (Acari: Tetranychidae) to sulfoxaflor stress

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2024-12-25 DOI:10.1016/j.cropro.2024.107100

Tianrong Xin, Xinyan Kong, Chenyu Jiang, Zhenzhen Li, Zhiwen Zou, Bin Xia

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引用次数: 0

Abstract

Panonychus citri, is a destructive pest in citrus orchards. The diversity of pests in citrus orchards leads to the improper pesticides application, and induced resistance may be an alternative mechanism for pest resurgence. In the present study, the acute toxicity of sulfoxaflor against P. citri adult females in leaf-dipping exposure experiment was determined at 24 h. Furthermore, the effects of sublethal (LC30) and lethal (LC50) concentrations of sulfoxaflor by the age-stage, two-sex life table approach were elucidated and these results revealed that the pre-adult period and the longevity of P. citri in the F1 generation were decreased. Additionally, the fecundity, R0 and r was significantly increased in the LC30- treated group, while the fecundity, R0 and λ of LC50- treated group was decreased significantly. Furthermore, the mRNA expression levels and activities of vitellogenin (Vg), vitellogenin receptor (VgR), carboxylesterases (CarE), glutathione-S-transferases (GST), and cytochrome P450 monooxygenases (CYP450) were significantly different at the sulfoxaflor treatments as compared to the control. Importantly, silencing of the CYP392B6 gene by RNA interference altered adult females of P. citri sensitivity towards sulfoxaflor and resulted in a significant increase in CYP450 content. Therefore, all these results in this study will contribute to a comprehensive risk assessment of sulfoxaflor on P. citri resurgence and will provide a convincing reference for the understanding of the mechanisms of fecundity and metabolic resistance to sulfoxaflor.

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柑桔全爪螨（螨目：叶螨科）对亚砜氟胁迫的生殖和解毒反应

柑橘全爪螨（Panonychus citri）是柑橘果园的一种破坏性害虫。柑桔园害虫的多样性导致施用不当，诱导抗性可能是害虫复生的另一种机制。本研究测定了亚砜对柑桔成虫雌虫浸叶暴露24 h后的急性毒性，并通过年龄期、两性寿命表法分析了亚砜浓度（LC30）和致死浓度（LC50）对柑桔成虫F1代前期和寿命的影响。LC30处理组的繁殖力、R0和r显著升高，LC50处理组的繁殖力、R0和λ显著降低。此外，与对照组相比，亚砜处理的卵黄原蛋白（Vg）、卵黄原蛋白受体（VgR）、羧酸酯酶（CarE）、谷胱甘肽- s转移酶（GST）和细胞色素P450单加氧酶（CYP450） mRNA表达量和活性均有显著差异。重要的是，通过RNA干扰沉默CYP392B6基因改变了成年雌性柑橘对亚砜的敏感性，导致CYP450含量显著增加。因此，本研究的结果将有助于全面评估亚砜对柑桔复生的风险，并为了解亚砜对柑桔繁殖力和代谢抗性的机制提供有说服力的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.