Impact of Magneto-Mechanical Actuation on Cell Differentiation: A Study Using Wireless, 3D-Printed Device and a Porous Ferrogel

Abstract

Cells perceive external and internally generated forces of different kinds, significantly impacting their cellular biology. In the relatively nascent field of mechanobiology, the impact of such forces is studied and further utilized to broaden the knowledge of cellular developmental pathways, disease progression, tissue engineering, and developing novel regenerative strategies. However, extensive considerations of mechanotransduction pathways for biomedical applications are still broadly limited due to a lack of affordable technologies in terms of devices and simple magnetic actuatable materials. Herein, synthesizing a monophasic, macroporous, in situ-fabricated gelatin-based ferrogel is reported using polyethylene glycol (PEG) coated-iron oxide (magnetite) particles with high magnetization. Developing a 3D printed, compact, and wireless device capable of providing a wide range of magneto-mechanical actuation using magnetic field susceptible materials in a noncontact manner is reported. Using the device and ferrogel, C2C12 myoblast differentiation is studied under magnetic field actuation, and significant differences in the myogenin, a differentiation marker, expression behavior are observed. Due to careful design considerations, robust component selection, and easy availability of low-cost precursor for magnetic responsive material fabrication, the device-ferrogel combination can be easily adapted to routine biological studies, thereby helping mechanobiology to be utilized for developing exciting new biomedical strategies.