{"title":"A rapid and reproducible method for generating germ-free <i>Drosophila melanogaster</i>.","authors":"An-Qi Li, Sha-Sha Li, Hui-Lin Li, Lan-Lan Zhong, Guo-Bao Tian, Xin-Yuan Zhao, Qiao-Ping Wang","doi":"10.52601/bpr.2024.240025","DOIUrl":null,"url":null,"abstract":"<p><p>Microbial communities exert a profound influence on various facets of animal behavior and physiology, making the comprehension of their interactions with hosts or the environment essential. <i>Drosophila melanogaster</i>, a widely recognized model organism, has been pivotal in elucidating host-microbe interactions. Despite the existence of several protocols for generating germ-free (GF) <i>Drosophila</i>, their reproducibility has been constrained by the technical difficulty of maintaining airtight conditions in centrifuge tubes. In this study, we introduce a refined method for the production of GF <i>Drosophila</i>, complemented by a straightforward verification process to ascertain its efficacy. We propose an innovative strategy employing bio-reaction tubes equipped with a 0.22 μm filter membrane cap, which facilitates the rearing and maintenance of GF flies, thereby streamlining the procedure and enhancing the efficiency of model construction.</p>","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 6","pages":"364-368"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693503/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.52601/bpr.2024.240025","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Microbial communities exert a profound influence on various facets of animal behavior and physiology, making the comprehension of their interactions with hosts or the environment essential. Drosophila melanogaster, a widely recognized model organism, has been pivotal in elucidating host-microbe interactions. Despite the existence of several protocols for generating germ-free (GF) Drosophila, their reproducibility has been constrained by the technical difficulty of maintaining airtight conditions in centrifuge tubes. In this study, we introduce a refined method for the production of GF Drosophila, complemented by a straightforward verification process to ascertain its efficacy. We propose an innovative strategy employing bio-reaction tubes equipped with a 0.22 μm filter membrane cap, which facilitates the rearing and maintenance of GF flies, thereby streamlining the procedure and enhancing the efficiency of model construction.
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