Expression and correlation of the long noncoding RNA PDIA3P and FAK/TGF-β pathway in oral submucous fibrosis rats.

IF 2.2 3区医学 Q2 Dentistry

Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2025-01-03 DOI:10.1016/j.jormas.2025.102214

Fan di Zhu, Yu Xuan Li, Ru Qian Guo, Yun Xu

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引用次数: 0

Abstract

Oral Submucous Fibrosis constitutes a severe condition affecting the lining of the mouth, which carries a risk of developing into oral squamous cell carcinoma, and seriously threaten individuals' lives. Currently, no specific treatment has been utilized to prevent OSF. The detailed mechanism of OSF is still elusive, and this study aims to clarify the mechanism of OSF.

Methods: This study was conducted to elucidate the molecular mechanisms of the long noncoding RNA PDIA3P (lncRNA PDIA3P) and FAK/TGF-β pathway in OSF. The OSF mice model was established by injecting with injected with 1000 mg/L arecoline solution, then the blood samples and oral submucosal tissues were collected from all groups of rats for further testing. HE staining, immunofluorescence, Western blot, and qRT-PCR were then used to elucidate the molecular mechanisms of the molecular mechanisms of the lncRNA PDIA3P and FAK/TGF-β pathway in OSF.

Results: The results showed that upregulation of the FAK/TGF-β signaling pathway can accelerate OSF process, while the downregulation of the FAK/TGF-β signaling pathway can prevent the OSF process. Similarly, enhanced activity of the lncRNA PDIA3P is implicated with OSF process, while reducing its level can served as a preventative method against the OSF development.

Conclusion: Upregulating the lncRNA PDIA3P and FAK/TGF-β pathway can accelerate the process of OSF.

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长链非编码RNA PDIA3P与FAK/TGF-β通路在口腔黏膜下纤维化大鼠中的表达及相关性

口腔黏膜下纤维化是一种影响口腔内壁的严重疾病，有发展为口腔鳞状细胞癌的风险，严重威胁个体的生命。目前，还没有专门的治疗方法来预防OSF。OSF的具体机制尚不清楚，本研究旨在阐明OSF的机制。方法：本研究旨在阐明长链非编码RNA PDIA3P （lncRNA PDIA3P）和FAK/TGF-β通路在OSF中的分子机制。通过注射1000 mg/L槟榔碱溶液建立OSF小鼠模型，然后采集各组大鼠血液和口腔黏膜下组织进一步检测。随后采用HE染色、免疫荧光、Western blot、qRT-PCR等方法对OSF中lncRNA PDIA3P和FAK/TGF-β通路的分子机制进行了研究。结果：结果表明，上调FAK/TGF-β信号通路可加速OSF过程，下调FAK/TGF-β信号通路可阻止OSF过程。同样，lncRNA PDIA3P活性的增强与OSF过程有关，而降低其水平可以作为预防OSF发展的方法。结论：上调lncRNA PDIA3P和FAK/TGF-β通路可加速OSF的发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Stomatology Oral and Maxillofacial Surgery Dentistry-Oral Surgery

CiteScore

2.20

自引率

9.10%

发文量

305

期刊介绍： J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics. Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses. All manuscripts submitted to the journal are subjected to peer review by international experts, and must: Be written in excellent English, clear and easy to understand, precise and concise; Bring new, interesting, valid information - and improve clinical care or guide future research; Be solely the work of the author(s) stated; Not have been previously published elsewhere and not be under consideration by another journal; Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.