Extractive optimization of bioactive compounds in aerial parts of cuphea carthagenensis using Box-Behnken experimental design.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-01-05 DOI:10.1080/14786419.2024.2448845

Nilza Alves de Oliveira Jacobucci, M M Pinc, Mariana Dalmagro, Joice Karina Otênio Ribeiro, Talita Anielle de Assunção, Elissandro Jair Klein, Edson Antônio da Silva, Paula Derksen Macruz, Ezilda Jacomassi, Odair Alberton, Jaqueline Hoscheid

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引用次数: 0

Abstract

Cuphea carthagenensis is known for its potential antioxidant and anti-inflammatory properties, attributed to triterpenes, flavonoids, and tannins. This study aimed to optimise the extraction process for aerial parts of C. carthagenensis using vortex extraction. A Box-Behnken experimental design coupled with response surface methodology was employed to evaluate the effects of three independent variables: sample-to-solvent ratio, velocity, and temperature. The optimised extract underwent phytochemical screening and assessment of its antimicrobial capacity. The highest extraction yields were obtained through maceration (22.84%) and ultrasound-assisted extraction (21.95%). However, the extract by vortex extraction exhibited significantly higher phenolic content (250.17 µg_EAG g_ext^-1) and antioxidant capacity. The optimal extraction conditions were identified as a sample-to-solvent ratio of 1:35, 12,000 rpm, and a temperature of 60 °C. The optimised extract was characterised by a predominance of glycosylated flavonoids and demonstrated moderate antibacterial activity, particularly against Streptococcus pyogenes, Staphylococcus epidermidis, and Bacillus subtilis.

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采用Box-Behnken实验设计优化龙参地上部生物活性成分的提取。

Cuphea carthagenensis因其潜在的抗氧化和抗炎特性而闻名，归因于三萜，类黄酮和单宁。本研究旨在优化旋涡提取法提取红花地上部分的工艺条件。采用Box-Behnken实验设计结合响应面法，对样品与溶剂比、速度和温度三个自变量的影响进行了评价。优化后的提取物进行了植物化学筛选和抗菌能力评估。浸出率最高（22.84%），超声辅助提取率最高（21.95%）。而涡旋提取液的酚类物质含量（250.17µgEAG）和抗氧化能力显著提高。最佳提取条件为料液比1:35，转速12000 rpm，提取温度60℃。优化后的提取物具有糖基化黄酮类化合物的优势，并表现出适度的抗菌活性，特别是对化脓性链球菌、表皮葡萄球菌和枯草芽孢杆菌。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.