ESCRT elicits vacuolar fission in the absence of Vps4 in budding yeast

IF 2.5 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Most Naoshia Tasnin , Tsuneyuki Takuma , Yuka Takahashi , Takashi Ushimaru
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引用次数: 0

Abstract

In budding yeast, endosomal sorting complex required for transport (ESCRT) mediates microautophagy by vacuolar membrane invagination into the vacuolar lumen, followed by Vps4-assisted membrane constriction and abscission. Here, we show that ESCRT elicits vacuolar fission in the absence of Vps4 after nutrient starvation, although vacuolar fusion is facilitated in wild-type cells in these conditions. ESCRT mediated vacuolar membrane invagination in vps4Δ cells, thereby causing vacuolar fission. It is known that vacuolar fission requires phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) and β-propellers that bind polyphosphoinositides (PROPPINs), PI(3,5)P2-binding proteins. However, PROPPIN, but not PI(3,5)P2, was dispensable for the ESCRT-mediated vacuolar fragmentation. Finally, we showed evidence that microlipophagy triggers vacuolar fission. Thus, disruption of the coordinated sequence of ESCRT–Vps4 operations in microautophagy leads to vacuolar fragmentation. This study provides insight into the ESCRT–Vps4 axis-dependent cellular disfunctions and related diseases.
在芽殖酵母中没有Vps4的情况下,ESCRT诱导液泡裂变。
在出芽酵母中,运输所需的内体分选复合体(ESCRT)通过空泡膜内陷到空泡腔中介导微自噬,然后是vps4辅助的膜收缩和脱落。在这里,我们证明了ESCRT在缺乏Vps4的营养饥饿后引发空泡裂变,尽管在这些条件下野生型细胞的空泡融合更容易。ESCRT介导vps4Δ细胞的空泡膜内陷,从而引起空泡裂变。众所周知,液泡裂变需要磷脂酰肌醇3,5-二磷酸(PI(3,5)P2)和β-螺旋桨结合多磷酸肌醇(PROPPINs), PI(3,5)P2结合蛋白。然而,对于escrt介导的液泡破裂,PROPPIN而非PI(3,5)P2是必不可少的。最后,我们发现了微脂肪吞噬触发空泡裂变的证据。因此,微自噬中ESCRT-Vps4操作协调序列的破坏导致液泡断裂。这项研究为ESCRT-Vps4轴依赖性细胞功能障碍和相关疾病提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biochemical and biophysical research communications
Biochemical and biophysical research communications 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
1400
审稿时长
14 days
期刊介绍: Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics
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