Plasmonic Nanodiamond – Microneedle Bioinspired System for Ultrarapid Sampling and Quantum Sensing of Melanoma-Related MiRNA

IF 18.5 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Min Wu, Lei Liu, Youqiang Xing, Ze Wu, Peng Huang, Bingjue Li
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引用次数: 0

Abstract

MicroRNA (miRNA) in interstitial fluid (ISF) is a promising biomarker for early disease diagnosis, but its quantification is challenging due to low sampling efficiency and weak signals. Here, inspired by the osmotic pressure-driven feeding mechanism of sea anemones, a novel is designed that plasmonic nanodiamonds – microneedles (PND-MNs) bioinspired system for ultrarapid sampling and quantum sensing of melanoma-related miRNA. Specifically, high-osmotic hydrogel microneedles, prepared by immersing in LiCl solution, exhibit a substantial swelling ratio for rapid ISF enrichment, extracting 0.92 ± 0.14 mg within 5 min. Concurrently, by coating fluorescent nanodiamonds (FNDs) with hollow composite metal Au-Ag NPs, precise modulation of the fluorescence intensity of nitrogen vacancy color centers (NV-center) in FNDs has been achieved. This innovative quantum sensing technology significantly enhances the fluorescence signal in the PND-MNs bioinspired system, amplifying it by a factor of 16, reducing the detection limit of miRNA to an unprecedented 0.68 fm (to the knowledge, no method based on fluorescent microneedles for detecting biomarkers has surpassed this level). Furthermore, the PND-MNs bioinspired system, employing backpropagation neural network (BPNN), achieves high accuracy in recognizing miRNA concentrations (R2 = 0.9986). By combining rapid sampling, signal enhancement, and machine learning, it holds promise for detecting ISF biomarkers, offering significant clinical applications.

Abstract Image

等离子体纳米金刚石-微针生物启发系统用于黑色素瘤相关MiRNA的超快速采样和量子传感
间质液(ISF)中的MicroRNA (miRNA)是一种很有前景的早期疾病诊断生物标志物,但由于采样效率低和信号弱,其定量具有挑战性。在此,受海葵渗透压驱动的进食机制的启发,设计了一种新的等离子体纳米金刚石微针(PND-MNs)生物启发系统,用于黑色素瘤相关miRNA的超快速采样和量子传感。其中,浸在LiCl溶液中制备的高渗透水凝胶微针具有较高的膨胀率,可快速富集ISF,在5分钟内提取0.92±0.14 mg。同时,通过在荧光纳米金刚石(fnd)表面包裹空心复合金属Au-Ag NPs,实现了fnd中氮空位色中心(nv中心)荧光强度的精确调制。这种创新的量子传感技术显著增强了PND-MNs生物激发系统中的荧光信号,将其放大了16倍,将miRNA的检测限降低到前所未有的0.68 fm(据了解,基于荧光微针检测生物标志物的方法还没有超过这一水平)。此外,采用反向传播神经网络(BPNN)的PND-MNs生物启发系统在识别miRNA浓度方面取得了很高的准确性(R2 = 0.9986)。通过结合快速采样、信号增强和机器学习,它有望检测ISF生物标志物,提供重要的临床应用。
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来源期刊
Advanced Functional Materials
Advanced Functional Materials 工程技术-材料科学:综合
CiteScore
29.50
自引率
4.20%
发文量
2086
审稿时长
2.1 months
期刊介绍: Firmly established as a top-tier materials science journal, Advanced Functional Materials reports breakthrough research in all aspects of materials science, including nanotechnology, chemistry, physics, and biology every week. Advanced Functional Materials is known for its rapid and fair peer review, quality content, and high impact, making it the first choice of the international materials science community.
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