Impact of automated nucleic acid extraction platforms on plasma Cytomegalovirus DNA loads quantitated by real-time PCR normalized to the 1st WHO international standard.

Enfermedades infecciosas y microbiologia clinica (English ed.) Pub Date : 2025-01-01 DOI:10.1016/j.eimce.2024.07.007

Ángela Sánchez-Simarro, Eliseo Albert, Paula Michelena, Estela Giménez, David Navarro

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Abstract

Introduction: The extent to which commercially available nucleic acid extraction platforms impact the magnitude of Cytomegalovirus (CMV) DNA loads measured in plasma specimens by 1st WHO standard-normalized real-time PCR assays is uncertain.

Methods: This retrospective study compares the performance of Abbott m2000sp, Qiagen QIAsymphony SP, and KingFisher Flex platforms using plasma samples from allogeneic hematopoietic stem cell transplant recipients and plasma spiked with the CMV AD169 strain. The Abbott RealTime CMV PCR assay was used for CMV DNA quantitation.

Results: Maximum differences in CMV DNA loads quantified in plasma from 11 allo-HSCT and spiked plasma over a wide range of viral DNA concentrations (2.0-4.0 log₁₀ IU/ml) were ≤0.5 log₁₀ IU/ml.

Conclusions: The CMV DNA extraction efficiency of the platforms evaluated varies. The impact of these variations on CMV DNA loads quantified in plasma may not be clinically relevant.

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自动核酸提取平台对实时荧光定量PCR检测血浆巨细胞病毒DNA载量的影响。

商业上可获得的核酸提取平台对第一次WHO标准标准化实时PCR测定血浆标本中巨细胞病毒（CMV） DNA载量的影响程度尚不确定。方法：本回顾性研究比较了雅培m2000sp、Qiagen QIAsymphony SP和KingFisher Flex平台使用同种异体造血干细胞移植受体血浆样本和CMV AD169毒株加标血浆的性能。采用Abbott RealTime CMV PCR检测CMV DNA定量。结果：11例同种异体造血干细胞移植和加标血浆中CMV DNA载量在病毒DNA浓度范围（2.0-4.0 log10 IU/ml）内的最大差异≤0.5 log10 IU/ml。结论：不同平台对CMV DNA的提取效率存在差异。这些变化对血浆中量化的巨细胞病毒DNA负荷的影响可能与临床无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Enfermedades infecciosas y microbiologia clinica (English ed.)

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