Biosynthesis and health promoting traits of green synthesized cobalt oxide nanoparticles.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-01-03 DOI:10.1038/s41598-024-82679-y

Raghda Moawad, Yasmine Abdallah, Mohamed Mohany, Salim S Al-Rejaie, Sinisa Djurasevic, Mohamed Fawzy Ramadan, Ahmed Bakr Mousa

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Abstract

Nanomedical applications have increased significantly. This work aimed to fabricate and characterize cobalt oxide nanoparticles (CoOnps) synthesized biologically via aqueous Alhagi maurorum extract and evaluate their cytotoxic and antimicrobial impacts. Green-synthesized CoOnps were prepared and analyzed using UV-Vis spectrophotometer UV-vis, Scanning electron microscopy (SEM), Transmission electron microscopy TEM, Energy dispersive X-ray analysis EDAX, Fourier transform infrared, FTIR, and X-ray diffraction (XRD). In vitro traits of green-synthesized CoOnps were studied on ovarian cancer cells (SKOV3) using a Sulforhodamine B (SRB) method. The cytotoxic effect and IC50 were estimated. Moreover, concentrations of 10, 30, 40, 70, 100, 200, 300, 400 and 500 μg/mL CoOnps were applied to investigate their antimicrobial effect against Listeria, Staphylococcus aureus and Streptococcus as gram +ve pathogenic bacteria, Bifidobacterium bifidum 2203, Bifidobacterium bifidum LMG 10,645, Bifidobacterium breve LMC 017, Bifidobacterium angulatum 2238 and Bifidobacterium longum ATCC 15,707 as probiotics, E. coli as gram -ve bacterial model and yeast strain Candida albicans. CoOnps showed anti-ovarian cancer effects at 24.02 μg/mL. Furthermore, it exerted antimicrobial activity versus Listeria, Streptococcus, S. aureus, and E. coli were 31.66 ± 0.88, 24.33 ± 2.08, 25.66 ± 0.33, and 33.00 ± 6.08; however, they did not suppress the growth of Candida albicans and all tested Bifidobacterial strains up to concentrations of 500 μg/mL with significant difference compared to all concentrations p < 0.05. Green synthesis of CoOnps is a low-cost, eco-friendly and easily prepared method. Its impressive features as cytotoxic SKOV3, a cell line ovarian cancer and antibacterial effect for some gram +ve and -ve bacteria, besides maintaining probiotics, could candidate them as competitive agents for medical, pharmacological, agricultural and food applications.

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绿色合成氧化钴纳米颗粒的生物合成及其健康促进特性。

纳米医学应用显著增加。本研究的目的是制备和表征氧化钴纳米颗粒（CoOnps），并评估其细胞毒性和抗菌作用。采用紫外可见分光光度计（UV-Vis）、扫描电镜（SEM）、透射电镜（TEM）、能量色散x射线分析（EDAX）、傅里叶变换红外、FTIR和x射线衍射（XRD）对绿色合成的CoOnps进行了分析。采用磺胺B （Sulforhodamine B， SRB）法研究了绿色合成的CoOnps在卵巢癌细胞（SKOV3）上的体外性状。估计细胞毒作用和IC50。采用浓度为10、30、40、70、100、200、300、400和500 μg/mL的CoOnps分别对致病菌李斯特菌、金黄色葡萄球菌和链球菌、益生菌双歧杆菌2203、双歧杆菌LMG 10645、短双歧杆菌LMC 017、角双歧杆菌2238和长双歧杆菌ATCC 15707、模型细菌大肠杆菌和酵母菌白色念珠菌的抑菌效果进行了研究。CoOnps浓度为24.02 μg/mL，具有抗卵巢癌作用。对李斯特菌、链球菌、金黄色葡萄球菌和大肠杆菌的抑菌活性分别为31.66±0.88、24.33±2.08、25.66±0.33和33.00±6.08；然而，当浓度达到500 μg/mL时，它们对白色念珠菌和所有被测双歧杆菌菌株的生长没有抑制作用，与所有浓度相比差异显著p

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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