Low-Intensity Pulsed Ultrasound Delays the Onset of Osteoporosis and Dyslipidemia in Mice With Premature Ovarian Insufficiency

IF 2.1 4区医学 Q2 ACOUSTICS

Journal of Ultrasound in Medicine Pub Date : 2025-01-04 DOI:10.1002/jum.16641

Juan Deng MMed, Junfen Li BS, Xinbin Han BE, Yiqing Zhou BS, Sicheng He BS, Jie Xu BS, Juan Qin PhD, Guolin Song MMed, Xinfang Xiao MMed, Liu Wu MMed, Yan Wang PhD

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引用次数: 0

Abstract

Objectives

The pathogenesis of premature ovarian insufficiency (POI) not only affects the ovarian structure and function but also gives rise to complications such as osteoporosis and dyslipidemia. Although low-intensity pulsed ultrasound (LIPUS) has been proven effective in treating POI, its impact on the associated complications remains unexplored. Therefore, this study aims to investigate the effects of LIPUS irradiation on osteoporosis and dyslipidemia in a mouse model of POI.

Methods

The post-treatment complications following LIPUS cessation were monitored in POI rats at 30 and 120 days in this trial conducted by us. The secretion of follicle-stimulating hormone (FSH) was assessed using enzyme-linked immunosorbent assay (ELISA) at different time points in POI rats. Pathological changes in the liver and femur were observed through HE staining. Blood lipid and bone metabolism indexes were measured via blood biochemical testing. Osteoporosis was evaluated using MicroCT and Masson staining techniques. The expression level of inflammatory factors in bone tissue was detected by Western blot (WB) test.

Results

The serum FSH content showed a significant decrease in the LIPUS group on day 0, day 15, and day 30 (P < .05). On the 30th day following LIPUS treatment, TC, TG, and LDL-C decreased in the LIPUS group while HDL-C increased with no statistically significant differences (P > .05); the indexes of femur parameters (BS/TV, BV/TV, Tb.Th, BMD, and TMD) were increased (P > .05). On the 120th day after LIPUS treatment, TC content demonstrated a significant decrease (P < .05), and TG and LDL-C displayed a downward trend (P > .05) while HDL-C content increased (P < .05); the femur parameters were significantly reduced (P < .05). The LIPUS group exhibited an increased presence of new fibrillar fibers. Levels of IL-6 and IL-1β significantly decreased with LIPUS treatment, whereas osteocalcin (OCN) expression notably increased (P < .05).

Conclusions

The application of LIPUS demonstrates potential in mitigating complications associated with POI in rats through the reduction of FSH secretion levels and inhibition of tissue inflammation, thereby presenting a promising avenue for women with POI to explore more efficacious treatment alternatives.

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低强度脉冲超声延缓卵巢功能不全小鼠骨质疏松和血脂异常的发生。

目的：卵巢功能不全（POI）的发病机制不仅影响卵巢的结构和功能，还可引起骨质疏松、血脂异常等并发症。虽然低强度脉冲超声（LIPUS）已被证明对治疗POI有效，但其对相关并发症的影响尚不清楚。因此，本研究旨在探讨LIPUS照射对POI小鼠模型骨质疏松和血脂异常的影响。方法：对POI大鼠在30d和120d停用LIPUS后的并发症进行监测。采用酶联免疫吸附试验（ELISA）测定POI大鼠不同时间点促卵泡激素（FSH）的分泌水平。HE染色观察肝、股骨的病理改变。通过血液生化检测血脂和骨代谢指标。使用MicroCT和Masson染色技术评估骨质疏松症。Western blot （WB）检测骨组织中炎症因子的表达水平。结果：LIPUS组患者血清FSH含量在第0天、第15天、第30天均显著降低（P < 0.05）；股骨参数指标(BS/TV、BV/TV、Tb；Th、BMD、TMD)增高（P < 0.05）。在LIPUS治疗后第120天，TC含量显著降低（P < 0.05），而HDL-C含量升高（P < 0.05）。结论：LIPUS通过降低FSH分泌水平和抑制组织炎症，有可能减轻POI大鼠的并发症，为POI女性患者探索更有效的治疗方案提供了一条有希望的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Ultrasound in Medicine 医学-核医学

CiteScore

5.10

自引率

4.30%

发文量

205

审稿时长

1.5 months

期刊介绍： The Journal of Ultrasound in Medicine (JUM) is dedicated to the rapid, accurate publication of original articles dealing with all aspects of medical ultrasound, particularly its direct application to patient care but also relevant basic science, advances in instrumentation, and biological effects. The journal is an official publication of the American Institute of Ultrasound in Medicine and publishes articles in a variety of categories, including Original Research papers, Review Articles, Pictorial Essays, Technical Innovations, Case Series, Letters to the Editor, and more, from an international bevy of countries in a continual effort to showcase and promote advances in the ultrasound community. Represented through these efforts are a wide variety of disciplines of ultrasound, including, but not limited to: -Basic Science- Breast Ultrasound- Contrast-Enhanced Ultrasound- Dermatology- Echocardiography- Elastography- Emergency Medicine- Fetal Echocardiography- Gastrointestinal Ultrasound- General and Abdominal Ultrasound- Genitourinary Ultrasound- Gynecologic Ultrasound- Head and Neck Ultrasound- High Frequency Clinical and Preclinical Imaging- Interventional-Intraoperative Ultrasound- Musculoskeletal Ultrasound- Neurosonology- Obstetric Ultrasound- Ophthalmologic Ultrasound- Pediatric Ultrasound- Point-of-Care Ultrasound- Public Policy- Superficial Structures- Therapeutic Ultrasound- Ultrasound Education- Ultrasound in Global Health- Urologic Ultrasound- Vascular Ultrasound