Antimicrobial Efficacy of Cloxacillin-Loaded Chitosan Nanoparticles Against Staphylococcus aureus Biofilms in Subclinical Mastitis.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-01-03 DOI:10.1007/s00284-024-04030-y

Mohammadsaeed Eskandari, Zohreh Abdolmaleki, Farhad Moosakhani, Mohammad Amin Eslampour

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Abstract

Bovine mastitis is the most widespread disease that causes financial loss in the dairy industry. Staphylococcus aureus is a well-researched multidrug-resistant opportunistic bacterium that is frequently linked to subclinical mastitis and causes significant economic losses. A further problem in the management of S. aureus infections is its capacity to form biofilms; bacteria inside biofilms exhibit greater resistance to antimicrobials than planktonic cells. The most effective method for controlling mastitis is antibiotic therapy. Cloxacillin (CLX), ampicillin, and ceftiofur are currently the most often utilized drying treatments for dairy cattle. We have evaluated the therapy efficiency of cloxacillin-loaded chitosan nanoparticles (CLX-CS NPs) as well as the relationship between biofilm production, gene profile and the type of trial group (CLX and CLX-CS NPs) against S.aureus isolated from milk samples of cows diagnosed with subclinical mastitis. Investigation of phenotypic biofilm production showed that majority of the S. aureus isolates extracted from milk were producers of biofilm. Cloxacillin-loaded chitosan nanoparticles were able to significantly decrease the MIC (p < 0.05) and MBC values compared to cloxacillin. The relationship between the gene profile and the types of trial groups shows that coating cloxacillin with chitosan nanoparticles (CS-NPs) was able to reduce the MIC and MBC value in all nine gene profile groups. These findings indicate that administration of cloxacillin-loaded CS-NPs in the treatment of mastitis may improve cloxacillin therapeutic properties and could act as a potential alternative to the cloxacillin antibiotic for the treatment of bovine mastitis.

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载氯沙西林壳聚糖纳米颗粒对亚临床乳腺炎金黄色葡萄球菌生物膜的抗菌作用。

牛乳腺炎是造成奶牛业经济损失最普遍的疾病。金黄色葡萄球菌是一种经过深入研究的多重耐药机会性细菌，经常与亚临床乳腺炎有关，并造成重大经济损失。金黄色葡萄球菌感染管理中的另一个问题是其形成生物膜的能力；与浮游细胞相比，生物膜内的细菌对抗菌剂表现出更强的抗药性。控制乳腺炎最有效的方法是抗生素治疗。氯唑西林（CLX）、氨苄西林和头孢噻呋是目前奶牛最常用的干燥疗法。我们评估了负载氯唑西林的壳聚糖纳米颗粒（CLX-CS NPs）的治疗效果，以及生物膜生成、基因图谱和试验组（CLX 和 CLX-CS NPs）类型对从诊断为亚临床乳腺炎的奶牛奶样中分离出的金黄色葡萄球菌的作用。对生物膜产生表型的调查表明，从牛奶中提取的大多数金黄色葡萄球菌都能产生生物膜。添加了氯唑西林的壳聚糖纳米颗粒能够显著降低金黄色葡萄球菌的 MIC（p

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.