Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli.

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

BMC Research Notes Pub Date : 2025-01-03 DOI:10.1186/s13104-024-07033-x

Rania Abozahra, Dina Shlkamy, Sarah M Abdelhamid

{"title":"Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli.","authors":"Rania Abozahra, Dina Shlkamy, Sarah M Abdelhamid","doi":"10.1186/s13104-024-07033-x","DOIUrl":null,"url":null,"abstract":"Objectives: The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination.Results: ɸEcM-vB1 bacteriophage exhibited high lytic activity against different MDR E. coli isolates and showed stability over wide pH and temperature range. It belongs to the Myoviridae family of the caudovirales order according to TEM. It had a latent period of 5 min and an average burst size of 271.72 pfu/cell. Genomic analysis revealed that it is susceptible to digestion by EcoRI. Ten structural proteins were detected by SDS-PAGE. ɸEcM-vB1 is considered a promising candidate for phage therapy applications.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"3"},"PeriodicalIF":1.6000,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699686/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Research Notes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13104-024-07033-x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination.

Results: ɸEcM-vB1 bacteriophage exhibited high lytic activity against different MDR E. coli isolates and showed stability over wide pH and temperature range. It belongs to the Myoviridae family of the caudovirales order according to TEM. It had a latent period of 5 min and an average burst size of 271.72 pfu/cell. Genomic analysis revealed that it is susceptible to digestion by EcoRI. Ten structural proteins were detected by SDS-PAGE. ɸEcM-vB1 is considered a promising candidate for phage therapy applications.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

期刊介绍： BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.