Ricardo Barbosa Sousa, Alessandra Cristina Dametto, Gabriela Fontana de Mesquita, Pedro Henrique Ricardo, Júlia Venturini Helaehil, Santiago Medina-Carrasco, Josy Anteveli Osajima Furtini, Guilherme Ferreira Caetano, Hernane S Barud, Edson C Silva-Filho
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引用次数: 0
Abstract
Bacterial nanocellulose (BNC) has attracted considerable attention in the field of biomedical engineering due to its potential for use in bone regeneration applications. The present study investigates the in vitro and in vivo efficacy of bacterial nanocellulose (BNC) combined with calcium and cerium ions (BNC-Ce:CaP) in bone regeneration applications. XRD analysis confirmed the presence of monetite and hydroxyapatite phases in BNC-CaP, while BNC-Ce:CaP revealed an additional brushite phase. Based on XPS analysis, cerium (III) is found in BNC-Ce:CaP at a concentration of 4.14 % (mol/mol). BNC revealed ultrafine 3D nanofibers with diameters ranging from 20.8 to 53.0 nm, while BNC-Ce:CaP composite, containing cerium, exhibited urchin-like structures with diameters around 1 µm and BNC-CaP composite presented phosphates covering the fiber surfaces, leading to significant thickness increases and pleat formation (70-180 nm). The composite materials demonstrated insignificant cytotoxicity. The results performed by histomorphometric analysis demonstrated that the BNC-Ce:CaP composites showed superior mineralized tissue formation after 60 days. Gene expression revealed a reduction in the inflammatory response and an increase in the expression of osteogenic markers, such as Bmp-2 and Osterix, in addition to an increase in the expression of angiogenic genes, such as Vegf. These findings highlight the potential of BNC-Ce:CaP composites as effective barriers to promote bone regeneration.
期刊介绍：
Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields.
Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication.
The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.
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