LIPUS activated piezoelectric pPLLA/SrSiO3 composite scaffold promotes osteochondral regeneration through P2RX1 mediated Ca2+ signaling pathway

IF 12.8 1区 医学 Q1 ENGINEERING, BIOMEDICAL
Chengxiao Liu , Bin Yu , Zhaowenbin Zhang , Lefeng Su , Ruiqing Wang , Yu Jin , Weiming Guo , Ruomei Li , Zhen Zeng , Peng Mei , Jiang Chang , Lunguo Xia , Chen Yang , Bing Fang
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Abstract

Addressing the concurrent repair of cartilage and subchondral bone presents a significant challenge yet is crucial for the effective treatment of severe joint injuries. This study introduces a novel biodegradable composite scaffold, integrating piezoelectric poly-l-lactic acid (pPLLA) with strontium-enriched silicate bioceramic (SrSiO3). This innovative scaffold continually releases bioactive Sr2+ and SiO32− ions while generating an electrical charge under low-intensity pulsed ultrasound (LIPUS) stimulation, a clinically recognized method. The scaffold's unique dual action, emanating both chemical and electrical signals, activates the purinergic receptor P2X 1 (P2RX1) calcium ion channel, promoting an influx of intracellular calcium ions. This process results in a synergistic enhancement of both chondrogenic activities of rat chondrocytes (rCCs) and osteogenic activities of rat bone marrow mesenchymal stem cells (rBMSCs). Furthermore, the scaffold's effectiveness in integrating articular cartilage and subchondral bone repair is confirmed in a rat model of joint osteochondral injury. This study thereby offers a groundbreaking approach for treating severe osteoarticular cartilage defects.

Abstract Image

LIPUS激活的压电式pPLLA/SrSiO3复合支架通过P2RX1介导的Ca2+信号通路促进骨软骨再生。
解决软骨和软骨下骨的同时修复是一项重大挑战,但对于有效治疗严重关节损伤至关重要。本研究介绍了一种新型的可生物降解复合支架,将压电聚乳酸(pPLLA)与富锶硅酸盐生物陶瓷(SrSiO3)集成在一起。这种创新的支架不断释放生物活性的Sr2+和SiO32-离子,同时在低强度脉冲超声(LIPUS)刺激下产生电荷,这是一种临床公认的方法。支架独特的双重作用,发出化学和电信号,激活嘌呤能受体P2X 1 (P2RX1)钙离子通道,促进细胞内钙离子的流入。这一过程导致大鼠软骨细胞(rCCs)的成软骨活性和大鼠骨髓间充质干细胞(rBMSCs)的成骨活性协同增强。此外,该支架在关节软骨损伤大鼠模型中证实了其整合关节软骨和软骨下骨修复的有效性。因此,这项研究为治疗严重的骨关节软骨缺损提供了一种突破性的方法。
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来源期刊
Biomaterials
Biomaterials 工程技术-材料科学:生物材料
CiteScore
26.00
自引率
2.90%
发文量
565
审稿时长
46 days
期刊介绍: Biomaterials is an international journal covering the science and clinical application of biomaterials. A biomaterial is now defined as a substance that has been engineered to take a form which, alone or as part of a complex system, is used to direct, by control of interactions with components of living systems, the course of any therapeutic or diagnostic procedure. It is the aim of the journal to provide a peer-reviewed forum for the publication of original papers and authoritative review and opinion papers dealing with the most important issues facing the use of biomaterials in clinical practice. The scope of the journal covers the wide range of physical, biological and chemical sciences that underpin the design of biomaterials and the clinical disciplines in which they are used. These sciences include polymer synthesis and characterization, drug and gene vector design, the biology of the host response, immunology and toxicology and self assembly at the nanoscale. Clinical applications include the therapies of medical technology and regenerative medicine in all clinical disciplines, and diagnostic systems that reply on innovative contrast and sensing agents. The journal is relevant to areas such as cancer diagnosis and therapy, implantable devices, drug delivery systems, gene vectors, bionanotechnology and tissue engineering.
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