ATG8 delipidation is not universally critical for autophagy in plants

IF 14.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Yong Zou, Jonas A. Ohlsson, Sanjana Holla, Igor Sabljić, Jia Xuan Leong, Florentine Ballhaus, Melanie Krebs, Karin Schumacher, Panagiotis N. Moschou, Simon Stael, Suayib Üstün, Yasin Dagdas, Peter V. Bozhkov, Elena A. Minina
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Abstract

Intracellular recycling via autophagy is governed by post-translational modifications of the autophagy-related (ATG) proteins. One notable example is ATG4-dependent delipidation of ATG8, a process that plays critical but distinct roles in autophagosome formation in yeast and mammals. Here, we aim to elucidate the specific contribution of this process to autophagosome formation in species representative of evolutionarily distant green plant lineages: unicellular green alga Chlamydomonas reinhardtii, with a relatively simple set of ATG genes, and a vascular plant Arabidopsis thaliana, harboring expanded ATG gene families. Remarkably, the more complex autophagy machinery of Arabidopsis renders ATG8 delipidation entirely dispensable for the maturation of autophagosomes, autophagic flux, and related stress tolerance; whereas autophagy in Chlamydomonas strictly depends on the ATG4-mediated delipidation of ATG8. Importantly, we also demonstrate the distinct impact of different Arabidopsis ATG8 orthologs on autophagosome formation, especially prevalent under nitrogen depletion, providing new insight into potential drivers behind the expansion of the ATG8 family in higher plants. Our findings underscore the evolutionary diversification of the molecular mechanism governing the maturation of autophagosomes in eukaryotic lineages and highlight how this conserved pathway is tailored to diverse organisms.

Abstract Image

在植物自噬中,ATG8的脱脂作用并不是普遍的关键
细胞内自噬循环是由自噬相关蛋白的翻译后修饰控制的。一个值得注意的例子是ATG8的atg4依赖性脱脂,这一过程在酵母和哺乳动物的自噬体形成中起着关键但独特的作用。在这里,我们的目的是阐明这一过程对进化上遥远的绿色植物谱系的代表物种自噬体形成的具体贡献:单细胞绿藻莱茵衣藻(Chlamydomonas reinhardtii)具有一组相对简单的ATG基因,以及维管植物拟南芥(Arabidopsis thaliana)具有扩展的ATG基因家族。值得注意的是,拟南芥更复杂的自噬机制使得ATG8脱脂对于自噬体的成熟、自噬通量和相关的胁迫耐受性完全没有作用;而衣藻的自噬完全依赖于atg4介导的ATG8的脱脂作用。重要的是,我们还证明了不同拟南芥ATG8同源基因对自噬体形成的独特影响,特别是在氮耗尽下普遍存在,为高等植物ATG8家族扩张背后的潜在驱动因素提供了新的见解。我们的发现强调了在真核生物谱系中控制自噬体成熟的分子机制的进化多样化,并强调了这种保守途径是如何适应不同生物的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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