Bioinformatics analysis of miR-2861 and miR-5011-5p that function as potential tumor suppressors in colorectal carcinogenesis.

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY
Alper Aytekin, Hikmet Kadakal, Deniz Mihcioglu, Turkan Gurer
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引用次数: 0

Abstract

Background: The study aimed to was to investigate the relationship between miR-2861, miR-5011-5p, and colorectal carcinogenesis.

Method: In the present study, it was isolated RNA from both the tumor and non-tumor tissue of a total of 80 CRC patients and after synthesizing the cDNA, it was performed qRT-PCR to determine the expression levels of miR‑2861 and miR‑5011-5p. In addition, it was predicted that dysregulated miRNAs targets, pathways and functional gene annotations that may be important in colorectal carcinogenesis using KEGG pathway and GO analysis.

Results: The resulting data revealed that both expression levels of miR-2861 and miR-5011-5p were significantly decreased in tumor tissues compared with non-tumor tissues of CRC patients. The GO and KEGG pathway analysis showed that miR-2861 and miR-5011-5p may participate in multiple the biological process, cellular components, and molecular function subcategories such as mitotic cell cycle, regulation of small GTPase mediated signal transduction, cell death, and acid binding transcription factor activity. It was also revealed that target genes of miRNAs can be found in signaling pathways such as TGF-beta, Rap1, Ras, cAMP, Wnt, mTOR and, PI3K-Akt signaling pathways.

Conclusion: These findings imply that miR-2861 and miR-5011-5p might function as tumor suppressors in the development of CRC.

结直肠癌发生中作为潜在肿瘤抑制因子的miR-2861和miR-5011-5p的生物信息学分析。
背景:本研究旨在探讨miR-2861、miR-5011-5p与结直肠癌发生的关系。方法:本研究共从80例结直肠癌患者的肿瘤和非肿瘤组织中分离RNA,合成cDNA后,采用qRT-PCR检测miR - 2861和miR - 5011-5p的表达水平。此外,通过KEGG通路和GO分析,预测了mirna的失调靶点、途径和功能基因注释可能在结直肠癌发生中起重要作用。结果:所得数据显示,与非肿瘤组织相比,CRC患者肿瘤组织中miR-2861和miR-5011-5p的表达水平均显著降低。GO和KEGG通路分析表明,miR-2861和miR-5011-5p可能参与有丝分裂细胞周期、调节小GTPase介导的信号转导、细胞死亡和酸结合转录因子活性等多种生物学过程、细胞组分和分子功能亚类。研究还发现,mirna的靶基因可在tgf - β、Rap1、Ras、cAMP、Wnt、mTOR、PI3K-Akt等信号通路中发现。结论:这些发现提示miR-2861和miR-5011-5p可能在结直肠癌的发展中起抑癌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
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