Modulating lysophospholipids with Paraoxonase-1: Exploring its impact on inflammatory responses and immune reactions

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical and biophysical research communications Pub Date : 2025-02-01 DOI:10.1016/j.bbrc.2024.151234

Takuya Shimura , Hideaki Isago , Yoshifumi Morita , Ryunosuke Ohkawa , Naoyuki Yoshikawa , Yoshikazu Ono , Makoto Kurano

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引用次数: 0

Abstract

Introduction

Paraoxonase-1 (PON1) is a crucial esterase in cardiovascular health, closely associated with HDL and known for its antioxidant and anti-inflammatory properties. Reduced PON1 activity has been linked to cardiovascular diseases. Lysophospholipids (LysoPLs), essential for cellular processes and immune responses, are implicated in the pathogenesis of cardiovascular diseases and are bound to lipoproteins, contributing to their diverse effects. Thus, we hypothesize that the relationship between PON1 and cardiovascular diseases may involve the modulation of LysoPLs by PON1. This study aims to investigate how PON1 potentially influences LysoPLs.

Methods

We quantified the levels of LysoPLs in HepG2 cells by using liquid chromatography-mass spectrometry, manipulating PON1 expression or knockdown.

Results

In cells overexpressing PON1, there was a significant increase in cellular levels of lysophosphatidylserine (LysoPS) and medium levels of LysoPS. Conversely, in cells with PON-1 knockdown, cellular levels of lysophosphatidylcholine (LysoPC) and medium levels of LysoPC showed a significant decrease.

Conclusions

PON1 is involved in modulating LysoPLs, which contribute to the antioxidant and anti-inflammatory properties of HDL, often attributed to PON1.

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用对氧磷酶-1调节溶血磷脂：探讨其对炎症反应和免疫反应的影响。

对氧磷酶-1 （PON1）是心血管健康中至关重要的酯酶，与HDL密切相关，并以其抗氧化和抗炎特性而闻名。PON1活性降低与心血管疾病有关。溶血磷脂（lysopl）是细胞过程和免疫反应所必需的，与心血管疾病的发病机制有关，并与脂蛋白结合，有助于其多种作用。因此，我们假设PON1与心血管疾病之间的关系可能涉及PON1对lysopl的调节。本研究旨在探讨PON1如何潜在地影响lysopl。方法：采用液相色谱-质谱联用，操纵PON1表达或敲低，定量测定HepG2细胞中lysopl的水平。结果：在过表达PON1的细胞中，细胞内溶血磷脂酰丝氨酸（LysoPS）水平和溶血磷脂酰丝氨酸（LysoPS）水平显著升高。相反，在PON-1敲低的细胞中，细胞中溶血磷脂酰胆碱（LysoPC）和LysoPC水平显著降低。结论：PON1参与调节溶索酚，溶索酚有助于HDL的抗氧化和抗炎特性，通常归因于PON1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics