Kinetics of charge-dependent reversible condensation of reflectin nanostructures†

IF 5.2 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Materials Advances Pub Date : 2024-11-25 DOI:10.1039/D4MA00788C

Iana Lychko, Cátia Lopes Soares, Arménio Jorge Moura Barbosa, Tomás Rosa Calmeiro, Rodrigo Ferrão de Paiva Martins, Ana Margarida Gonçalves Carvalho Dias and Ana Cecília Afonso Roque

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Abstract

Reflectins are unique cephalopod proteins found in specialized cells. They form fast triggerable nanostructures in vivo that play a crucial role in light reflection and camouflage. We investigated the rapid kinetics of in vitro reversible self-assembly of two recombinant reflectin sequences (R1b and R6) using pH variations as a trigger. By employing experimental and theoretical approaches across scales, we demonstrated that R6 exhibits superior reversibility and faster assembly kinetics. R6 maintained reversible assembly for up to 7 rapid pH cycles, with changes occurring in less than 20 minutes. This enhanced performance is attributed to R6's higher content of pH-sensitive residues and favorable charge distribution. Our findings impact the design of reflectin-inspired artificial biophotonic systems, offering potential applications in sensors, adaptive optics, and dynamic display technologies.

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反射蛋白纳米结构的电荷依赖可逆缩合动力学

反射蛋白是在特殊细胞中发现的独特的头足类蛋白质。它们在体内形成快速触发的纳米结构，在光反射和伪装中起着至关重要的作用。我们研究了两个重组反射蛋白序列（R1b和R6）在体外可逆自组装的快速动力学，以pH变化为触发因素。通过采用跨尺度的实验和理论方法，我们证明了R6具有优越的可逆性和更快的组装动力学。R6在7个快速pH循环中保持可逆组装，变化在不到20分钟内发生。这种性能的提高是由于R6具有较高的ph敏感残基含量和良好的电荷分布。我们的发现影响了反射激发的人工生物光子系统的设计，在传感器、自适应光学和动态显示技术方面提供了潜在的应用。

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