Micropore Resistance Counting Platform for Multiplexed and Ultrasensitive Detection of Mycotoxins and Biomarkers

IF 15.8 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Nano Pub Date : 2025-01-03 DOI:10.1021/acsnano.4c12394

Letian Li, Mengjiao Wang, Yongzhen Dong, Deyang Yu, Yiping Chen

{"title":"Micropore Resistance Counting Platform for Multiplexed and Ultrasensitive Detection of Mycotoxins and Biomarkers","authors":"Letian Li, Mengjiao Wang, Yongzhen Dong, Deyang Yu, Yiping Chen","doi":"10.1021/acsnano.4c12394","DOIUrl":null,"url":null,"abstract":"Development of a multiplexed and sensitive biosensing platform is a priority for public health security. We report a micropore resistance counting platform based on polystyrene microsphere size-based encoding and <i>Clostridium butyricum</i> Argonaute (CbAgo) decoding for multiplexed and ultrasensitive detection. Initially, we constructed a target DNA-modified polystyrene microsphere coding system based on micropore resistance counting. Subsequently, the precise recognition and cleavage capabilities of the guide DNA-activated CbAgo protein enable the decoding of the encoded microsphere system. Changes in the concentration of polystyrene microspheres are presented as a signal readout. The platform demonstrated excellent performance in multiplexed detection of three mycotoxins (with a sensitivity range over 4 orders of magnitude reaching the pg/mL level) and two inflammatory markers at pg/mL. Combining precise enzyme cleavage by CbAgo with micropore resistance counting, the developed platform is a multiplexed and highly sensitive detection tool with wide-ranging potential in applications such as clinical diagnosis, food safety inspection, and environmental monitoring.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"153 1","pages":""},"PeriodicalIF":15.8000,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Nano","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acsnano.4c12394","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Development of a multiplexed and sensitive biosensing platform is a priority for public health security. We report a micropore resistance counting platform based on polystyrene microsphere size-based encoding and Clostridium butyricum Argonaute (CbAgo) decoding for multiplexed and ultrasensitive detection. Initially, we constructed a target DNA-modified polystyrene microsphere coding system based on micropore resistance counting. Subsequently, the precise recognition and cleavage capabilities of the guide DNA-activated CbAgo protein enable the decoding of the encoded microsphere system. Changes in the concentration of polystyrene microspheres are presented as a signal readout. The platform demonstrated excellent performance in multiplexed detection of three mycotoxins (with a sensitivity range over 4 orders of magnitude reaching the pg/mL level) and two inflammatory markers at pg/mL. Combining precise enzyme cleavage by CbAgo with micropore resistance counting, the developed platform is a multiplexed and highly sensitive detection tool with wide-ranging potential in applications such as clinical diagnosis, food safety inspection, and environmental monitoring.

Abstract Image

查看原文本刊更多论文

多路超灵敏检测真菌毒素和生物标志物的微孔阻力计数平台

开发多路、灵敏的生物传感平台是公共卫生安全的优先事项。我们报道了一个基于聚苯乙烯微球尺寸编码和丁酸梭菌解码的微孔电阻计数平台，用于多路复用和超灵敏检测。首先，我们构建了一个基于微孔阻力计数的靶dna修饰聚苯乙烯微球编码系统。随后，引导dna激活的CbAgo蛋白的精确识别和切割能力使编码的微球系统能够解码。聚苯乙烯微球浓度的变化以信号读出的形式呈现。该平台在3种真菌毒素（灵敏度范围超过4个数量级，达到pg/mL）和2种炎症标志物（pg/mL）的多重检测中表现出优异的性能。该平台将CbAgo精确酶切与微孔电阻计数相结合，是一种多路、高灵敏度的检测工具，在临床诊断、食品安全检测和环境监测等领域具有广泛的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Nano 工程技术-材料科学：综合

CiteScore

26.00

自引率

4.10%

发文量

1627

审稿时长

1.7 months

期刊介绍： ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.