Increase the amyloid pathology in meningitis-induced cognitive impairment: sex differences

IF 13 1区 医学 Q1 CLINICAL NEUROLOGY
Lucineia Danielski, Vijayasree V Giridharan, Ngozi Iwunze, Celso Catumbela, Rodrigo Morales, Tatiana Barichello
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Abstract

Background

Pneumococcal meningitis is a type of meningitis that may face long-term neurological complications, leading to the hypothesis that it might contribute to the deposition of beta-amyloid (Aβ) and predispose individuals to Alzheimer’s pathology.

Method

Male and female APP/PS1 mice, 50 days old, were divided into control (n = 5) and meningitis (n = 6). Under anesthesia, an intracisternal injection of either artificial cerebrospinal fluid (CSF) as a placebo or 5 × 109 colony-forming units (CFU) of S. pneumoniae suspension was injected. Meningitis was confirmed through culture incubation. The Open Field Test (OFT) and Novel Object Recognition (NOR) tasks were conducted when the mice reached 180 days of age; after this, the hippocampus and prefrontal cortex (PFC) were stained to be evaluated by immunofluorescence.

Result

Mice of both sexes experiencing meningitis displayed cognitive impairment (p < 0,001) evaluated for NOR, but the distribution of Aβ burden differed between males and females. Males exhibited elevated Aβ predominantly in the hippocampus (p < 0,05), while females showed heightened Aβ levels exclusively in the PFC (p < 0,05). Interestingly, only females with meningitis showed increased levels of IBA-1, a marker for microglial reactivity, in both the hippocampus (p = 0,0048) and PFC (p = 0,0023) compared to their controls.

Conclusion

The distinct patterns observed in Aβ distribution and microglial activation between sexes suggest a potential association, indicating that meningitis can induce elevated Aβ deposition, with microglia potentially playing a role in this divergent response.

增加淀粉样蛋白病理在脑膜炎引起的认知障碍:性别差异
背景肺炎球菌性脑膜炎是一种可能面临长期神经系统并发症的脑膜炎,这导致了它可能导致β -淀粉样蛋白(a β)沉积并使个体易患阿尔茨海默病的假设。方法将50日龄的APP/PS1小鼠分为对照组(n = 5)和脑膜炎组(n = 6)。在麻醉下,腹腔内注射人工脑脊液(CSF)作为安慰剂或5 × 109菌落形成单位(CFU)肺炎链球菌悬液。经培养培养证实为脑膜炎。当小鼠达到180日龄时,进行开放场测试(OFT)和新目标识别(NOR)任务;在此之后,海马和前额叶皮质(PFC)染色,免疫荧光评估。结果男女患脑膜炎的小鼠均出现认知障碍(p <;0,001)评估了NOR,但男性和女性之间的Aβ负荷分布存在差异。雄鼠主要在海马区表现出Aβ升高(p <;0.05),而女性仅在PFC中显示Aβ水平升高(p <;0 05)。有趣的是,与对照组相比,只有患有脑膜炎的女性在海马(p = 0.0048)和PFC (p = 0.0023)中显示IBA‐1水平升高,IBA‐1是小胶质细胞反应性的标志。结论两性间a β分布和小胶质细胞激活的不同模式提示可能存在关联,表明脑膜炎可诱导a β沉积升高,而小胶质细胞可能在这种差异反应中发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Alzheimer's & Dementia
Alzheimer's & Dementia 医学-临床神经学
CiteScore
14.50
自引率
5.00%
发文量
299
审稿时长
3 months
期刊介绍: Alzheimer's & Dementia is a peer-reviewed journal that aims to bridge knowledge gaps in dementia research by covering the entire spectrum, from basic science to clinical trials to social and behavioral investigations. It provides a platform for rapid communication of new findings and ideas, optimal translation of research into practical applications, increasing knowledge across diverse disciplines for early detection, diagnosis, and intervention, and identifying promising new research directions. In July 2008, Alzheimer's & Dementia was accepted for indexing by MEDLINE, recognizing its scientific merit and contribution to Alzheimer's research.
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