Generation of a USP9Y knockout human embryonic stem cell line with CRISPR-Cas9 technology.

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-02-01 Epub Date: 2024-12-28 DOI:10.1016/j.scr.2024.103646

Sai Wei, Yuting Zhen, Chao Sun, Yanlin Ma, Qi Li, Luan Wen

引用次数: 0

Abstract

Human embryonic stem cell (hESC) lines are vital tools for studying gene function, disease modeling, and therapy. We generated a USP9Y knockout hESC line using CRISPR-Cas9 in the male-derived H1 line. Targeted deletion of the USP9Y gene was confirmed via PCR and sequencing. The modified line retained pluripotency markers, exhibited a normal karyotype, and differentiated into all three germ layers. This model provides a valuable platform for studying USP9Y's role in human development and male infertility, offering insights into related disorders and therapeutic potential.

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利用CRISPR-Cas9技术产生USP9Y基因敲除的人胚胎干细胞系。

人类胚胎干细胞（hESC）系是研究基因功能、疾病建模和治疗的重要工具。我们使用CRISPR-Cas9在男性衍生的H1细胞系中产生了USP9Y敲除hESC细胞系。通过PCR和测序证实了USP9Y基因的靶向缺失。修饰后的细胞系保留了多能性标记，核型正常，并分化为三个胚层。该模型为研究USP9Y在人类发育和男性不育中的作用提供了一个有价值的平台，为相关疾病和治疗潜力提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.