The effect of pipette- and laser-induced blastocyst collapse before vitrification on their re-expansion and clinical outcome after warming

IF 3.7 2区医学 Q1 OBSTETRICS & GYNECOLOGY

Reproductive biomedicine online Pub Date : 2025-02-01 DOI:10.1016/j.rbmo.2024.104476

Iris Martínez-Rodero , Borut Kovačič , Omar Shebl , Sabine Enengl , Julia Lastinger , Thomas Ebner

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引用次数: 0

Abstract

Research question

What are the effects of pipette- versus laser-assisted artificial blastocyst collapse (ABC) on the morphokinetics of warmed blastocyst re-expansion, and what is the potential effect on treatment outcomes?

Design

Surplus blastocysts were extracted from 203 patients. These were divided into three groups: study group A, artificial collapsed by the aspiration of blastocoel fluid with a pipette; study group B, trophectoderm opened with a laser pulse; control group, no manipulation before vitrification was performed. During the 5-year study period, 257 associated single-warm blastocyst transfers were scheduled. The start and duration of the re-expansion process before transfer were annotated. Pregnancy and live birth data were also collected for the transfers.

Results

The overall blastocyst survival rate was 96.9%, with no effect observed as a result of the two ABC methods. The re-expansion of blastocysts in study group B was initiated significantly sooner after warming (0.50 ± 0.37 h) than in group A (0.79 ± 0.56 h) or the control group (1.22 ± 1.00 h). The duration of the re-expansion process was significantly reduced in study groups A (P = 0.021) and B (P = 0.004) compared with the control group. The embryos of participants who achieved a live birth had a significantly (P < 0.001) faster start of re-expansion (0.60 ± 0.42 h) than the embryos in those who did not produce an ongoing pregnancy (1.05 ± 0.92 h).

Conclusions

Laser-treated blastocysts exhibited substantially shorter re-expansion times. Because faster re-expansion of the blastocyst is associated with positive treatment outcomes, the laser technique should be prioritized over the pipetting technique if ABC is considered.

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玻璃化前移液管和激光诱导囊胚塌陷对加热后囊胚再膨胀和临床结果的影响。

研究问题：移液管与激光辅助人工囊胚塌陷（ABC）对受热囊胚再膨胀的形态动力学有何影响？对治疗结果有何潜在影响？设计：从203例患者中提取剩余囊胚。研究对象分为三组：研究A组，用移液管抽吸囊胚液人工塌陷；B组，用激光脉冲打开滋养外胚层；对照组，玻璃化前不做任何操作。在5年的研究期间，共安排了257例相关的单温囊胚移植。对转移前再扩张过程的开始和持续时间进行了注释。妊娠和活产数据也被收集用于转移。结果：总囊胚存活率为96.9%，两种ABC方法对囊胚存活率均无影响。B组囊胚在加热后的再膨胀时间（0.50±0.37 h）明显早于A组（0.79±0.56 h）和对照组（1.22±1.00 h），再膨胀时间（P = 0.021）和B组（P = 0.004）明显短于对照组。活产的胚胎的再膨胀开始时间（0.60±0.42小时）显著（P < 0.001）快于未妊娠的胚胎（1.05±0.92小时）。结论：激光处理的囊胚的再膨胀时间大大缩短。由于囊胚快速再扩张与积极的治疗结果相关，如果考虑ABC，激光技术应优先于移液技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.