Proteomic analysis reveals anticancer mechanisms of Bhallataka taila in inhibiting lung cancer progression and metastasis.

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2025-01-02 DOI:10.1039/d4mo00156g

Suchitha G P, Akhila B Rai, Ravishankar Pervaje, Chinmaya Narayana Kotimoole, Prashant Kumar Modi, T S Keshava Prasad, Shobha Dagamajalu

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引用次数: 0

Abstract

Lung cancer remains the leading cause of cancer-related deaths worldwide due to its poor prognosis. Despite significant advancements in the understanding of cancer development, improvements in diagnostic methods, and multimodal therapeutic regimens, the prognosis of lung cancer has still not improved. Therefore, it is reasonable to look for newer and alternative medicines for treatment. Bhallataka nut extract, derived from the seeds of Semecarpus anacardium, is known for its anti-inflammatory and antioxidant properties, suggesting potential as a treatment for cancer. In this study, we investigated the molecular networks associated with the Bhallataka taila-mediated inhibition of lung adenocarcinoma. Treating lung cancer cell lines with Bhallataka taila resulted in decreased colony formation, proliferation, and migration, and increased apoptosis. Using a tandem mass tag (TMT)-based temporal quantitative proteomic analysis, we identified 173 overexpressed and 249 downregulated proteins among a total of 2879 proteins. Significantly altered proteins are associated with lung cancer progression, metastasis, invasion, migration, and epithelial-mesenchymal transition (EMT). The analysis of these altered proteins revealed molecular networks underlying the anticancer mechanisms of Bhallataka taila. Validation of these proteins and pathways affected by Bhallataka taila confirmed its utility in cancer treatment.

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蛋白质组学分析揭示了黑尾草抑制肺癌进展和转移的抗癌机制。

由于预后不良，肺癌仍然是世界范围内癌症相关死亡的主要原因。尽管对癌症发展的认识、诊断方法的改进和多模式治疗方案取得了重大进展，但肺癌的预后仍未得到改善。因此，寻找更新的替代药物进行治疗是合理的。巴拉塔卡坚果提取物，从半仙人掌种子中提取，以其抗炎和抗氧化特性而闻名，这表明它有治疗癌症的潜力。在这项研究中，我们研究了与巴拉塔卡草叶介导的肺腺癌抑制相关的分子网络。用巴罗塔卡芥治疗肺癌细胞系可减少菌落形成、增殖和迁移，增加细胞凋亡。利用基于串联质量标签（TMT）的时间定量蛋白质组学分析，我们在总共2879个蛋白中鉴定出173个过表达蛋白和249个下调蛋白。显著改变的蛋白与肺癌的进展、转移、侵袭、迁移和上皮-间质转化（EMT）有关。对这些改变的蛋白质的分析揭示了Bhallataka taila抗癌机制的分子网络。这些蛋白质和受巴拉塔卡泰拉影响的途径的验证证实了它在癌症治疗中的效用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.