Investigating the Impact of Selected B Vitamins (B1, B2, B6, and B12) on Acute Colitis Induced Experimentally in Rats.

IF 1.7 Q2 MEDICINE, GENERAL & INTERNAL
International Journal of Preventive Medicine Pub Date : 2024-11-28 eCollection Date: 2024-01-01 DOI:10.4103/ijpvm.ijpvm_232_23
Niloufar Dalayeli, Valiollah Hajhashemi, Ardeshir Talebi, Mohsen Minaiyan
{"title":"Investigating the Impact of Selected B Vitamins (B1, B2, B6, and B12) on Acute Colitis Induced Experimentally in Rats.","authors":"Niloufar Dalayeli, Valiollah Hajhashemi, Ardeshir Talebi, Mohsen Minaiyan","doi":"10.4103/ijpvm.ijpvm_232_23","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Malnutrition and lack of micronutrients are seen in many patients with ulcerative colitis (UC). Considering that vitamins play an important role in preventing or protecting against inflammation at the metabolic, physiological, and pathological levels, this research was aimed to investigate the effect of a number of vitamin B groups in improving UC.</p><p><strong>Methods: </strong>Experimental colitis was induced by rectal administration of acetic acid (3%) in male Wistar rats, and mega doses of thiamine (20 mg/kg), riboflavin (15 mg/kg), pyridoxine (30 mg/kg), and cyanocobalamin (250 μg/kg) alone or in combination were administered intraperitoneally for 5 days. In another group, normal dose (1/10 of the above-mentioned doses) of four vitamins in combination was administered for 15 days (started 10 days before colitis induction). Colon tissues were weighted and evaluated in terms of macroscopic, microscopic, and biochemical markers.</p><p><strong>Results: </strong>Normal dose of four vitamins in combination form and mega dose of thiamine caused a significant increase in the body weight of animals. All treatments except for pyridoxine (mega dose) diminished ulceration index, total colitis index, and colon weight compared to the control group. Myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels decreased significantly in all treatment groups.</p><p><strong>Conclusions: </strong>The anti-inflammatory and anti-ulcerative properties of selected B vitamins improved experimental colitis regardless of the dosage and duration of treatment. Despite its beneficial effect on biochemical markers, pyridoxine had the least effect in reducing the pathological features of colitis. More studies are needed to confirm the effect of these vitamins in the clinical setting of this disease.</p>","PeriodicalId":14342,"journal":{"name":"International Journal of Preventive Medicine","volume":"15 ","pages":"61"},"PeriodicalIF":1.7000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11687687/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Preventive Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ijpvm.ijpvm_232_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Malnutrition and lack of micronutrients are seen in many patients with ulcerative colitis (UC). Considering that vitamins play an important role in preventing or protecting against inflammation at the metabolic, physiological, and pathological levels, this research was aimed to investigate the effect of a number of vitamin B groups in improving UC.

Methods: Experimental colitis was induced by rectal administration of acetic acid (3%) in male Wistar rats, and mega doses of thiamine (20 mg/kg), riboflavin (15 mg/kg), pyridoxine (30 mg/kg), and cyanocobalamin (250 μg/kg) alone or in combination were administered intraperitoneally for 5 days. In another group, normal dose (1/10 of the above-mentioned doses) of four vitamins in combination was administered for 15 days (started 10 days before colitis induction). Colon tissues were weighted and evaluated in terms of macroscopic, microscopic, and biochemical markers.

Results: Normal dose of four vitamins in combination form and mega dose of thiamine caused a significant increase in the body weight of animals. All treatments except for pyridoxine (mega dose) diminished ulceration index, total colitis index, and colon weight compared to the control group. Myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels decreased significantly in all treatment groups.

Conclusions: The anti-inflammatory and anti-ulcerative properties of selected B vitamins improved experimental colitis regardless of the dosage and duration of treatment. Despite its beneficial effect on biochemical markers, pyridoxine had the least effect in reducing the pathological features of colitis. More studies are needed to confirm the effect of these vitamins in the clinical setting of this disease.

研究选定的B族维生素(B1、B2、B6和B12)对实验性大鼠急性结肠炎的影响。
背景:许多溃疡性结肠炎(UC)患者存在营养不良和微量营养素缺乏。考虑到维生素在代谢、生理和病理水平上都具有预防或保护炎症的重要作用,本研究旨在探讨多种维生素B组对UC的改善作用。方法:采用醋酸(3%)直肠灌胃诱导雄性Wistar大鼠实验性结肠炎,并腹腔注射大剂量硫胺素(20 mg/kg)、核黄素(15 mg/kg)、吡哆醇(30 mg/kg)、氰钴胺素(250 μg/kg),连续5 d。另一组以正常剂量(上述剂量的1/10)4种维生素联合用药15天(结肠炎诱导前10天开始)。根据宏观、微观和生化指标对结肠组织进行加权和评估。结果:正常剂量的四种维生素复合形式和大剂量的硫胺素可使动物体重显著增加。与对照组相比,除大剂量吡哆醇外的所有治疗均降低了溃疡指数、总结肠炎指数和结肠重量。髓过氧化物酶(MPO)活性和丙二醛(MDA)水平在所有治疗组均显著降低。结论:所选B族维生素的抗炎和抗溃疡特性改善实验性结肠炎,无论剂量和治疗时间如何。尽管吡哆醇对生化指标有有益的作用,但它在降低结肠炎病理特征方面的作用最小。需要更多的研究来证实这些维生素在这种疾病的临床环境中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
International Journal of Preventive Medicine
International Journal of Preventive Medicine MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
4.80%
发文量
107
期刊介绍: International Journal of Preventive Medicine, a publication of Isfahan University of Medical Sciences, is a peer-reviewed online journal with Continuous print on demand compilation of issues published. The journal’s full text is available online at http://www.ijpvmjournal.net. The journal allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository. The journal will cover technical and clinical studies related to health, ethical and social issues in field of Preventive Medicine. Articles with clinical interest and implications will be given preference.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信