Evaluating the Efficacy of Irisin Injection in Mimicking the Molecular Responses Induced by Endurance Exercise in Mouse Liver Tissue.

IF 1.7 Q2 MEDICINE, GENERAL & INTERNAL
International Journal of Preventive Medicine Pub Date : 2024-11-28 eCollection Date: 2024-01-01 DOI:10.4103/ijpvm.ijpvm_124_23
Shirin Shahabi, Fahimeh Esfarjani, Saeed Zamani, Fahimeh Zamani Rarani, Bahman Rashidi
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引用次数: 0

Abstract

Background: Physical activity has been found to improve liver health by reducing oxidative stress (OS), possibly through the protein irisin. Heat shock proteins (HSPs) and microRNAs (miRNAs) help regulate the body's response to stress and maintain cellular health. This study aimed to investigate the expression of the HSP70 gene and protein, miR-223a, and serum irisin levels in the liver after 8 weeks of endurance exercise or irisin injection.

Methods: Twenty-one mice were randomly assigned to a control group, an endurance training group, and an irisin injection group. The expression of the HSP70 gene and miR-223a was analyzed using real-time polymerase chain reaction (PCR), while HSP70 protein levels were measured using immunohistochemistry (IHC) and Western blot analysis. The concentration of irisin in the mouse serum was evaluated using the enzyme-linked immunosorbent assay (ELISA) method.

Results: The endurance training and irisin injection groups exhibited a significant increase in the HSP70 gene (405.30% and 816.03%, respectively) and protein expression (173.89% in IHC, 36.76% in Western blot for endurance training; 206.73% in IHC, 59.80% in Western blot for irisin injection) as well as elevated serum irisin levels (49.75% for endurance training and 60.65% for irisin injection) compared with the control group. In contrast, miR-223a expression decreased in both the endurance training (21.37%) and irisin injection (52.80%) groups (P < 0.05 in all cases). Mice in the irisin injection group demonstrated higher levels of the HSP70 gene (81.28%) and protein expression (11.99% in IHC and 16.84% in Western blot) and lower miR-223a levels (39.97%) than those in the endurance training group (P < 0.05).

Conclusions: The study concludes that irisin administration can replicate the effects of long-term endurance exercise on HSP70 and miR-223a and may have a more significant impact on their production than exercise training alone.

鸢尾素注射液对小鼠肝组织模拟耐力运动诱导的分子反应的效果评价。
背景:体育活动已被发现通过减少氧化应激(OS)来改善肝脏健康,可能是通过蛋白质鸢尾素。热休克蛋白(HSPs)和microrna (miRNAs)有助于调节身体对压力的反应并维持细胞健康。本研究旨在探讨耐力运动或鸢尾素注射8周后肝脏中HSP70基因和蛋白、miR-223a的表达以及血清鸢尾素的水平。方法:将21只小鼠随机分为对照组、耐力训练组和鸢尾素注射组。采用实时聚合酶链式反应(PCR)分析HSP70基因和miR-223a的表达,采用免疫组化(IHC)和Western blot分析HSP70蛋白水平。采用酶联免疫吸附法(ELISA)测定小鼠血清中鸢尾素的浓度。结果:耐力训练组和鸢尾素注射组HSP70基因和蛋白表达量显著升高(IHC组为173.89%,Western blot组为36.76%),分别为405.30%和816.03%;免疫组化组为206.73%,注射鸢尾素组为59.80%),血清鸢尾素水平升高(耐力训练组为49.75%,注射鸢尾素组为60.65%)。相比之下,在耐力训练组(21.37%)和鸢尾素注射组(52.80%)中,miR-223a的表达均下降(P < 0.05)。鸢尾素注射组小鼠HSP70基因(81.28%)和蛋白表达水平(免疫组化11.99%和Western blot 16.84%)高于耐力训练组(P < 0.05), miR-223a水平(39.97%)低于耐力训练组(P < 0.05)。结论:本研究得出结论,鸢尾素可以复制长期耐力运动对HSP70和miR-223a的影响,并且可能比单独运动训练对它们的产生有更显著的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Preventive Medicine
International Journal of Preventive Medicine MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
4.80%
发文量
107
期刊介绍: International Journal of Preventive Medicine, a publication of Isfahan University of Medical Sciences, is a peer-reviewed online journal with Continuous print on demand compilation of issues published. The journal’s full text is available online at http://www.ijpvmjournal.net. The journal allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository. The journal will cover technical and clinical studies related to health, ethical and social issues in field of Preventive Medicine. Articles with clinical interest and implications will be given preference.
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