Do plant biostimulants affect the survival of Escherichia coli in lettuce?

IF 4.1 2区 生物学 Q1 PLANT SCIENCES
Frontiers in Plant Science Pub Date : 2024-12-17 eCollection Date: 2024-01-01 DOI:10.3389/fpls.2024.1495463
Leonardo Fiore, Mariateresa Cardarelli, Maurizio Ruzzi, Anna Grazia Ficca, Youssef Rouphael, Francesca Luziatelli, Giuseppe Colla
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Abstract

Introduction: Considering that plant biostimulants can be sprayed multiple times on leafy crops even just before harvest, it is relevant to know the impact of biostimulant applications on Escherichia coli population dynamics of lettuce leaves to ensure food safety. Two trials were carried out to investigate whether the applications of a seaweed extract and a vegetal-derived protein hydrolysate (PH) could affect the E. coli growth in shake flasks (Exp. 1) and plant growth and survival of artificially inoculated E. coli on the leaf surface of lettuce grown in a floating system (Exp. 2).

Methods: The non-pathogenic E. coli strain K12 was used in both trials. In Exp. 1, biostimulants' inhibitory/stimulatory effect on E. coli growth was evaluated in liquid culture after 24 hours of incubation at 37°C. The 31-day agronomic trial (Exp. 2) was conducted in a polyethylene greenhouse on lettuce grown in a floating system.

Results: In Exp. 1, E. coli growth was not affected by LB medium amended with biostimulants, whereas both biostimulants stimulated total aerobic bacteria and inhibited E. coli population on lettuce leaves with a more pronounced inhibitory effect of PH applications on E. coli (Exp. 2). Total plant biomass and its partitioning (on fresh and dry weight basis), and N concentrations (as total N and nitrates) of leaves were not influenced by both biostimulant treatments.

Conclusion: The use of plant biostimulants could be a valuable and sustainable strategy to improve the microbiological quality of leafy greens to produce ready-to-eat foods.

植物生物刺激剂会影响生菜中大肠杆菌的存活吗?
导说:考虑到植物生物刺激素可以在收获前多次喷洒在多叶作物上,了解生物刺激素的施用对生菜叶片大肠杆菌种群动态的影响,以确保食品安全是有意义的。研究了海藻提取物和植物源蛋白水解物(PH)对摇瓶中大肠杆菌生长的影响(实验1),以及人工接种的大肠杆菌在漂浮系统生菜叶表面的生长和存活的影响(实验2)。方法:两项试验均采用非致病性大肠杆菌菌株K12。在实验1中,生物刺激剂在37℃培养24小时后对大肠杆菌生长的抑制/刺激作用进行了评估。为期31天的农艺试验(试验2)在聚乙烯温室中对漂浮系统种植的生菜进行了试验。结果:在实验1中,添加了生物刺激剂的LB培养基对大肠杆菌的生长没有影响,而两种生物刺激剂都刺激了生菜叶片上的总需氧细菌并抑制了大肠杆菌的数量,PH对大肠杆菌的抑制作用更为明显(实验2)。两种生物刺激剂处理对植物总生物量及其分配(以鲜重和干重为基础)和叶片的N浓度(以总N和硝酸盐为单位)没有影响。结论:植物性生物刺激素是提高绿叶蔬菜生产即食食品微生物质量的一种有价值且可持续的方法。
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来源期刊
Frontiers in Plant Science
Frontiers in Plant Science PLANT SCIENCES-
CiteScore
7.30
自引率
14.30%
发文量
4844
审稿时长
14 weeks
期刊介绍: In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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