Morin Ameliorates Myocardial Injury in Diabetic Rats via Modulation of Autophagy, Apoptosis, Inflammation, and Oxidative Stress.

IF 2.8 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy Pub Date : 2024-12-27 eCollection Date: 2024-01-01 DOI:10.2147/DMSO.S476867

Liping Zhu, Jizhong He

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引用次数: 0

Abstract

Background: Morin is a flavonol with beneficial effects on diabetic-related injuries. However, the effect of morin on diabetic cardiomyopathy and its association with autophagy, apoptosis, inflammation, and oxidative stress remains unclear. The current study aimed to reveal the mechanisms underlying morin-mediated protection against cardiac failure in diabetic rats.

Methods: Diabetic cardiomyopathy in albino Wistar rats was induced by streptozotocin (STZ). After treatment with a dose of 25, 50, and 100 mg/kg/day orally for the next 60 days, autophagic (p62, LC3, and BECN1), apoptotic (BCL2, CASP-3, and CASP9), inflammatory (IL-1β, IL-6, TNF-α), and oxidative stress (CAT, SOD, and MDA) markers in protein and gene levels as well as cardiac function tests were measured.

Results: The findings revealed that long-term morin treatment improved weight gain, lipid and glycemic profile, hypertension, and cardiac hypertrophy and fibrosis in diabetic rats compared to controls (p-value<0.001). Moreover, the upregulation of BCL-2, LC3, and BECN1 along with the downregulation of p62, CASP-3, and CASP-9 revealed that morin suppressed apoptosis and promoted autophagy in the cardiac tissue of rats with diabetes (p-value<0.05). Additionally, the reduction in IL-1β, IL-6, TNF-α, and MDA levels and the increment of SOD and CAT activity suggested that morin decreased inflammation and apoptosis in the heart of the rat models of diabetes (p-value<0.01).

Conclusion: These results may highlight the potential properties of morin as a therapeutic strategy for diabetic cardiomyopathy.

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马桑素通过调节自噬、凋亡、炎症和氧化应激改善糖尿病大鼠心肌损伤。

背景：桑辣素是一种黄酮醇，对糖尿病相关损伤有有益作用。然而，桑苷对糖尿病心肌病的作用及其与自噬、细胞凋亡、炎症和氧化应激的关系尚不清楚。本研究旨在揭示莫里素对糖尿病大鼠心脏衰竭的保护机制。方法：采用链脲佐菌素（STZ）诱导白化Wistar大鼠糖尿病性心肌病。在接下来的60天内，口服25、50和100 mg/kg/天的剂量后，测量自噬（p62、LC3和BECN1）、凋亡（BCL2、CASP-3和CASP9）、炎症（IL-1β、IL-6、TNF-α）和氧化应激（CAT、SOD和MDA）标志物的蛋白质和基因水平以及心功能测试。结果：研究结果显示，与对照组相比，长期治疗桑皮素可改善糖尿病大鼠的体重增加、血脂和血糖水平、高血压、心脏肥大和纤维化（p值）。结论：这些结果可能突出了桑皮素作为糖尿病心肌病治疗策略的潜在特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

5.90

自引率

6.10%

发文量

431

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed, open access, online journal. The journal is committed to the rapid publication of the latest laboratory and clinical findings in the fields of diabetes, metabolic syndrome and obesity research. Original research, review, case reports, hypothesis formation, expert opinion and commentaries are all considered for publication.