miR-141-3p inhibited BPA-induced proliferation and migration of lung cancer cells through PTGER4.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2025-02-01 Epub Date: 2024-12-29 DOI:10.1007/s10616-024-00692-5

Feng Ling, Wenbo Xie, Xiang Kui, Yuyin Cai, Meng He, Jianqiang Ma

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引用次数: 0

Abstract

The chemical substance bisphenol A (BPA) is widely used in household products, and its effect on human health has frequently been the focus of research. The aim of this study was to explore the potential molecular regulatory mechanism of BPA on the proliferation and migration of lung cancer cells. In this study, the H1299 and A549 lung cancer cell lines were selected as the study objects. The cells were treated with different concentrations of BPA (0, 0.1, 1, or 10 μM), and cell viability, proliferation, and migration were evaluated by CCK-8, EdU, clonogenic, and scratch test assays. Western blotting and RT‒qPCR were used to detect the expression of related proteins and genes. Our findings indicated that BPA markedly enhanced both the proliferation and migration capacities of lung cancer cells. In BPA-treated lung cancer cells, the level of miR-141-3p was decreased, PTGER4 expression was significantly increased, and PTGER4 knockdown reduced BPA-induced lung cancer cell proliferation and migration. In addition, miR-141-3p can target and negatively regulate the expression of PTGER4 and further inhibit PI3K/AKT signaling pathway activation and MMPs expression. Moreover, PTGER4 overexpression weakened the inhibitory effect of the miR-141-3p mimic on the proliferation and migration of lung cancer cells. In conclusion, miR-141-3p can inhibit the proliferation and migration of BPA-induced lung cancer cells by downregulating PTGER4, providing a new potential target for the treatment and prevention of lung cancer.

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-024-00692-5.

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miR-141-3p通过PTGER4抑制bpa诱导的肺癌细胞增殖和迁移。

化学物质双酚A （BPA）广泛应用于家庭用品中，其对人体健康的影响一直是人们关注的焦点。本研究旨在探讨双酚a对肺癌细胞增殖和迁移的潜在分子调控机制。本研究选择H1299和A549肺癌细胞系作为研究对象。用不同浓度的BPA（0、0.1、1或10 μM）处理细胞，通过CCK-8、EdU、克隆性和划痕试验评估细胞活力、增殖和迁移。Western blotting和RT-qPCR检测相关蛋白和基因的表达情况。我们的研究结果表明，BPA显著增强了肺癌细胞的增殖和迁移能力。在bpa处理的肺癌细胞中，miR-141-3p水平降低，PTGER4表达显著升高，PTGER4敲低可降低bpa诱导的肺癌细胞增殖和迁移。此外，miR-141-3p可以靶向并负调控PTGER4的表达，进而抑制PI3K/AKT信号通路的激活和MMPs的表达。此外，PTGER4过表达削弱了miR-141-3p模拟物对肺癌细胞增殖和迁移的抑制作用。综上所述，miR-141-3p可以通过下调PTGER4抑制bpa诱导的肺癌细胞的增殖和迁移，为治疗和预防肺癌提供了新的潜在靶点。补充信息：在线版本包含补充资料，可在10.1007/s10616-024-00692-5获得。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.